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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 8

sp. nov. and sp. nov., isolated from rivers in Southwest China No Access

Abstract

Eight Gram-stain-negative, aerobic, short rod-shaped and motile strains (DC21W, LYT5W, LYT10W, LYT16W, LYT22W, LYT23W, LYT24W and SH7W) were isolated from rivers in Southwest China. Comparisons based on the 16S rRNA gene sequences showed that strain DC21W shared the highest 16S rRNA gene sequence similarity (99.6 %) with 389, strain LYT5W shared 99.2 % with Npb-07, and the other isolated strains took DSM 3303 as their most similar strain, respectively. The phylogenetic trees reconstructed based on the 16S rRNA gene sequences also supported that strains 389, Npb-07 and DSM 3303 were the closest neighbours of the isolated strains. The phylogenomic tree showed similar phylogenetic relationships among these strains. The calculated OrthoANIu and digital DNA–DNA hybridization values among strains DC21W, LYT5W and other related strains were less than 93.7 and 53.7 %, respectively. The calculated OrthoANIu and digital DNA–DNA hybridization values among strains LYT10W, LYT16W, LYT22W, LYT23W, LYT24W, SH7W and DSM 3303 ranged from 94.8 to 97.2 % and from 59.8 to 74.9 %, respectively. Although these values were located in the transition region of species demarcation, their similar phenotypic, biochemical and genotypic characteristics supported that these six strains should be assigned to the species . Comparative genomic analyses showed that only DSM 3303 harboured 19 genes encoding the Type VI secretion system. Combining above descriptions, strains DC21W and LYT5W should represent two independent novel species of the genus , for which the names sp. nov. (type strain DC21W=GDMCC 1.3220=KCTC 92556) and sp. nov. (type strains LYT5W=GDMCC 1.3213=KCTC 92549) are proposed, respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): river and Vogesella
Funding
This study was supported by the:
  • Yunnan Provincial Ministry of Science and Technology (Award 202301AT070100)
    • Principle Award Recipient: HuibinLu
  • National Natural Science Foundation of China (Award 32060276)
    • Principle Award Recipient: LiChen
  • Yunnan Provincial Ministry of Science and Technology (Award 202203AC100002-02, 202005AF150005 and 202305AM070002)
    • Principle Award Recipient: GuangjieChen
© 2023 The Authors
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Vogesella aquatica sp. nov. and Vogesella margarita sp. nov., isolated from rivers in Southwest China
Int J Syst Evol Microbiol 73, 006011 (2023); https://doi.org/10.1099/ijsem.0.006011
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