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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 8

gen. nov., sp. nov., isolated from activated sludge No Access

Abstract

A bacterial strain designated as LB-31 was isolated from activated sludge. Strain LB-31 was Gram-stain-negative, non-spore-forming and rod-shaped with a single polar flagellum. The isolate grew at 15–37 °C, pH 5.5–8.5 and 0.5–8.5 % (w/v) NaCl. Genome sequencing revealed a genome size of 3.55 Mb, a G+C content of 61.1 mol% and 3222 protein-coding genes. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LB-31 formed a phyletic lineage that was distinct from the three known genus within the family . The 16S rRNA gene sequence similarity of strain LB-31 to all recognized bacterial species was no more than 94 %. Average nucleotide identity and digital DNA–DNA hybridization values between strain LB-31 and its phylogenetic neighbours were below 72 and 22 %, respectively. The average amino acid identity values for strain LB-31 and its closed phylogenetic neighbours and were below 63%, supporting that strain LB-31 was a member of a novel genus. The predominant respiratory quinone is Q-10, the fatty acid profile predominantly consisted of iso-C 9, iso-C, iso-C and summed feature 3 (comprising iso-C2-OH/C 7), and the major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The combined genotypic and phenotypic data show that strain LB-31 represents a novel species of a novel genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is LB-31 (=GDMCC 1.3625=KCTC 92687).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , Pseudokordiimonas caeni and taxonomy
Funding
This study was supported by the:
  • High-qualified Talents Scientific Research Startup Foundation of Nanyang Normal University (Award 2019ZX013)
    • Principle Award Recipient: NaLi
  • Major Scientific and Technological Special Project of Guizhou Province (Award BM2022019)
    • Principle Award Recipient: JianHe
  • Postdoctoral Research Foundation of China (Award 2021M701735)
    • Principle Award Recipient: NaLi
  • National Natural Science Foundation of China (Award 31900082)
    • Principle Award Recipient: NaLi
© 2023 The Authors
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2023-08-23
2024-12-06
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Pseudokordiimonas caeni gen. nov., sp. nov., isolated from activated sludge
Int J Syst Evol Microbiol 73, 006007 (2023); https://doi.org/10.1099/ijsem.0.006007
/content/journal/ijsem/10.1099/ijsem.0.006007
/content/journal/ijsem/10.1099/ijsem.0.006007
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