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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 8

sp. nov. and sp. nov., isolated from the respiratory tracts of marmots No Access

Abstract

Four Gram-stain-negative, oxidase-positive, non-motile, cocci-shaped bacteria strains (ZJ106, ZJ104, ZJ785 and ZJ930) were isolated from marmot respiratory tracts. Phylogenetic analyses based on 16S rRNA genes, 53 ribosomal protein sequences and 441 core genes supported that all four strains belonged to the genus with close relatives 10022 and ATCC 51483. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values were below the species-level thresholds (95–96 % for ANI, and 70 % for dDDH). The major fatty acids of all four strains were C 7 /C 6, C and C 9. Major polar lipids were composed of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. MK-8 was the major menaquinone. Based on Virulence Factor Database analysis, the four strains were found to contain NspA and PorB H-factor binding proteins that promote evasion of host immunity. Strains ZJ106 and ZJ104 contained structures similar to the capsule synthesis manipulator of . Based on phenotypic and phylogenetic evidence, we propose that strains ZJ106 and ZJ785 represent two novel species of the genus , respectively, with the names sp. nov. and sp. nov. The type strains are ZJ106 (=GDMCC 1.3111=JCM 35323) and ZJ785 (=GDMCC 1.1998=KCTC 82336)

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): capsule , Neisseria , polyphasic taxonomy and virulence genes
Funding
This study was supported by the:
  • Research Units of Discovery of Unknown Bacteria and Function (Award 2018RU010)
    • Principle Award Recipient: JianguoXu
  • National Key R&D Program of China (Award 2019TFC1200505)
    • Principle Award Recipient: LiyunLiu
  • National Key R&D Program of China (Award 2019TFC1200500)
    • Principle Award Recipient: JingYang
© 2023 The Authors
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2023-08-23
2024-05-08
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Neisseria lisongii sp. nov. and Neisseria yangbaofengii sp. nov., isolated from the respiratory tracts of marmots
Int J Syst Evol Microbiol 73, 006002 (2023); https://doi.org/10.1099/ijsem.0.006002
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