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Volume 73, Issue 8

sp. nov., isolated from No Access

Abstract

A Gram-stain-negative, rod-shaped, polar flagellated, aerobic, light-yellow bacterium, designated as 2012CJ41-6, was isolated from a sponge sample of from Chuja-myeon, Jeju-si, Jeju-do, Republic of Korea. On the basis of 16S rRNA gene sequencing, strain 2012CJ41-6 clustered with species of the genus and appeared closely related to . DSM 27839 (96.46 % similarity), . CECT 4357 (96.32 %), . ZGT108 (96.32 %), CECT 7639 (96.32 %) and . CECT 4292 (96.16 %). The average nucleotide identity and digital DNA–DNA hybridization between strain 2012CJ41-6 and the most closely related strain was 75.3 % and 19.6 %, indicating that 2012CJ41-6 represents a novel species of the genus . Growth occurred at 15–37 °C on marine medium in the presence of 0.5–10 % (w/v) NaCl and at pH 5.5–8.5. The DNA G+C content of the genomic DNA was 60.80 mol%, and ubiquinone-10 (Q-10) was the major respiratory quinone. The major cellular fatty acids (>5 %) were C 7 and/or C 6 (summed feature 8). The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, one unidentified phospholipid, one unidentified aminolipid, one unidentified aminophospholipid and five unidentified lipids. Physiological and biochemical characteristics indicated that strain 2012CJ41-6 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 2012CJ41-6 (=KACC 22645=LMG 32585).

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  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene sequence , polyphasic , Ruegeria spongiae and sponge
Funding
This study was supported by the:
  • Innovative Research Group Project of the National Natural Science Foundation of China (Award 2021R1I1A3046479)
    • Principle Award Recipient: Jin-SookPark
  • National Marine Biodiversity Institute of Korea
    • Principle Award Recipient: Jin-SookPark
© 2023 The Authors
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Ruegeria spongiae sp. nov., isolated from Callyspongia elongata
Int J Syst Evol Microbiol 73, 006001 (2023); https://doi.org/10.1099/ijsem.0.006001
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