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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 8

sp. nov., isolated from forest soil in Pyeongchang, Republic of Korea No Access

Abstract

Rod-shaped Gram-stain-negative, aerobic bacterial strains, designated PC14 and PC15, were isolated from a forest soil sample collected in Pyeongchang county, Gangwon-do, Republic of Korea. Strains PC14 and PC15 grew at 15–37 °C (optimum, 28–30 °C in tryptone soya agar and Mueller–Hinton agar), hydrolysed chitin and casein, and tolerated pH 8.5 and 2 % (w/v) NaCl. The strains were most closely related to members of the genus , namely DSM 3695 (98.4 %), Z29 (98.3 %), Gsoil 040 (97.8 %), MRP-15 (97.8 %) and JS16-4 (97.7 %). The type strain grew well on conventional commercial media in the laboratory, including tryptone soya agar, Mueller–Hinton agar, Reasoner's 2A agar, nutrient agar and Luria–Bertani agar. The major polar lipid profile comprised phosphatidylethanolamine, an unidentified aminolipid and unidentified polar lipids. The major respiratory quinone was menaquinone-7. The main fatty acids were iso-C, C ω5, C 3-OH, iso-C 3-OH and iso-C 3-OH. The DNA G+C content of the isolated strain based on the whole genome sequence was 46.6 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strains PC14 and PC15 and the reference type strains ranged from 71.0 to 76.5 %, and from 20.3 to 20.7 %, respectively. Based on phenotypic, chemotypic and genotypic evidence, strain PC15 could be differentiated phylogenetically and phenotypically from the recognized species of the genus . Therefore, strain PC15 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is PC15 (=KACC 22893=JCM 35788).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacteroidota , Chitinophaga nivalis , forest soil , novel species and Pyeongchang
Funding
This study was supported by the:
  • National Research Foundation of Korea (Award 2022R1A2C1010877)
    • Principle Award Recipient: JaisooKim
© 2023 The Authors
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2023-08-16
2024-05-08
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Chitinophaga nivalis sp. nov., isolated from forest soil in Pyeongchang, Republic of Korea
Int J Syst Evol Microbiol 73, 005981 (2023); https://doi.org/10.1099/ijsem.0.005981
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