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Abstract

A Gram-stain-negative, rod-shaped, non-motile and strictly aerobic bacterium, which showed biofilm-forming ability on polystyrene, designated as strain B-399, was isolated from the estuarine sediment of the Arakawa River near Tokyo Bay. It grew at pH 6.0–8.5, at 15–35 °C and in the presence of 0–7.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain B-399 was clustered in the genus and has 96.94 % sequence similarity to X12M-4, which was the only validly described species in this genus. On the basis of our genome sequencing analyses, the average nucleotide identity and digital DNA–DNA hybridization values between strains B-399 and X12M-4 were 79.54 and 22.30 %, respectively, which confirms that strain B-399 represents a novel species of the genus . The draft genome size and the DNA G+C content of strain B-399 were 4.12 Mb and 65.2 mol%, respectively. The major fatty acids (>10 %) of strain B-399 were C, summed feature 8 (C 6 and/or C 7) and C cyclo 8. The polar lipids were phosphatidylcholine, phosphatidylglycerol, an unidentified phospholipid, an unidentified aminolipid and unidentified lipids. The respiratory quinone was Q-10. These chemotaxonomic features were almost coincident with those of the genus . Therefore, strain B-399 should be classified as representing a new species of the genus , for which the name sp. nov. is proposed. The type strain is B-399 (=NBRC 115629=DSM 114148).

Funding
This study was supported by the:
  • Heiwa Nakajima Foundation
    • Principle Award Recipient: TakeshiTerahara
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/content/journal/ijsem/10.1099/ijsem.0.005969
2023-08-14
2024-07-23
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