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Volume 73, Issue 8

sp. nov., an extremely halophilic archaeon isolated from a hypersaline soil No Access

Abstract

An extremely halophilic archaeal strain, designated S1CR25-10, was isolated from hypersaline soil sampled in the Odiel Saltmarshes Natural Area in Southwestern Spain (Huelva) and subjected to a polyphasic taxonomic characterization. The cells were Gram-stain-negative, motile and their colonies were pink-pigmented. It was a strictly aerobic haloarchaeon that could grow at 25–55 °C (optimum, 37 °C), at pH 6.0–9.0 (optimum, pH 7.0–8.0) and in the presence of 12–30 % (w/v) total salts (optimum, 20–25 %, w/v). The phylogenetic analysis based on the comparison of the 16S rRNA gene sequences revealed that strain S1CR25-10 belongs to the genus , with 98.9 % similarity to SLN56. In addition, the values of orthologous average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity were below the threshold limits accepted for prokaryotic species delineation, with SLN56 showing the highest relatedness values (92.6 % and 48.4 %, respectively). The major polar lipids were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and a glycolipid chromatographically identical to sulfated diglycosyl diether. The DNA G+C content of the isolate was 63.8 mol%. Based on the phylogenetic, phenotypic and chemotaxonomic characterization and the whole genome results, strain S1CR25-10 represents a new species within the genus , for which the name sp. nov., with type strain S1CR25-10 (=CECT 30623=CCM 9251), is proposed.

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  • Accepted:
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Keyword(s): haloarchaea , hypersaline soil , Natrinema , taxogenomics and taxonomy
Funding
This study was supported by the:
  • Consejería de Economía, Innovación, Ciencia y Empleo, Junta de Andalucía (Award BIO-213)
    • Principle Award Recipient: AntonioVentosa
  • Consejería de Economía, Conocimiento, Empresas y Universidad, Junta de Andalucía (Award P20_01066)
    • Principle Award Recipient: AntonioVentosa
  • MCIN/AEI (Award PID2020-118136GB-I00)
    • Principle Award Recipient: AntonioVentosa
© 2023 The Authors
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2023-08-14
2024-05-09
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Natrinema salsiterrestre sp. nov., an extremely halophilic archaeon isolated from a hypersaline soil
Int J Syst Evol Microbiol 73, 005960 (2023); https://doi.org/10.1099/ijsem.0.005960
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