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Volume 73, Issue 8

sp. nov., a novel marine bacterium isolated from the Atlantic Ocean Open Access

Abstract

A bacterial strain, PhyBa_CO2_2, was isolated from the North Atlantic Gyre, offshore Terceira Island in the Azores. Initially, the NCBI nucleotide analysis based on 16S rRNA gene sequences revealed that the strain belongs to the genus , with a 100 % identity with LMG 19861. However, further genomic characterization through average nucleotide identity (ANI) and digital DNA–DNA hybridization analyses showed values of 96.06 and 64.80 %, respectively. Comparative genomics also highlighted differences in gene content. The genome size of PhyBa_CO2_2 is 3.6 Mbp and the DNA G+C content is 72.1 mol%. Chemotaxonomic analysis demonstrated that the composition of the fatty acids was mainly composed of anteiso-C (46.04 %), iso-C (13.70 %) and anteiso-C (9.48 %), and the polar lipids were mainly diphosphatidylglycerol, phosphatidylglycerol and two unidentified glycolipids. Furthermore, the diagnostic amino acid of the cell wall was -diaminopimelic acid and the predominant menaquinone was MK7. Finally, phenotypic analysis revealed differences in biochemical profiles between PhyBa_CO2_2 and its closely related strains in terms of indole production, urease and β-glucuronidase activity. Therefore, based on the genomic, chemotaxonomic and phenotypic data obtained, we concluded that strain PhyBa_CO2_2 represents a new species, for which the name sp. nov. is proposed in reference to its isolation site. The type strain is PhyBa_CO2_2 (=DSM 114113= CECT 30695).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Atlantic Ocean , Brachybacterium and marine bacteria
Funding
This study was supported by the:
  • Fundo Regional para a Ciência e Tecnologia (Award M3.1.a/F/017/2018)
    • Principle Award Recipient: SusanaRibeiro
  • Fundação para a Ciência e a Tecnologia (Award LA/P/0094/2020)
    • Principle Award Recipient: NotApplicable
  • Fundação para a Ciência e a Tecnologia (Award UIDB/50017/2020)
    • Principle Award Recipient: NotApplicable
  • Fundação para a Ciência e a Tecnologia (Award UIDP/50017/2020)
    • Principle Award Recipient: NotApplicable
  • ARM program-ENA project and IITAA (Award UIDB/00153/2019)
    • Principle Award Recipient: NotApplicable
  • Fundo Regional para a Ciência e Tecnologia (Award ACORES-01-0145-FEDER-000038)
    • Principle Award Recipient: NotApplicable
  • Fundação para a Ciência e a Tecnologia
    • Principle Award Recipient: VanessaOliveira
  • Fundação para a Ciência e a Tecnologia (Award UI/BD/150890/2021)
    • Principle Award Recipient: Inêsde Castro
  • Fundação para a Ciência e a Tecnologia (Award CEECIND/00070/2017)
    • Principle Award Recipient: FranciscoJ.R.C. Coelho
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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Brachybacterium atlanticum sp. nov., a novel marine bacterium isolated from the Atlantic Ocean
Int J Syst Evol Microbiol 73, 005959 (2023); https://doi.org/10.1099/ijsem.0.005959
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