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Volume 73, Issue 8

sp. nov. and sp. nov., isolated from sea foam in Queensland, Australia Open Access

Abstract

Strains USC-21046 and USC-21048 were isolated from foaming coastal marine waters on the Sunshine Coast, Queensland, Australia. Both strains displayed growth and morphological characteristics typical for members belonging to the genus . The major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine, and the major fatty acids were C, C ω9, C and C 10-methyl. The mycolic acids of strains USC-21046 and USC-21048 consisted of chain lengths between 50–64 and 56–68, respectively. Moreover, both of those strains contained -diaminopimelic acid and ribose, arabinose, glucose and galactose as whole cell sugars. Based on the phylogenomic results, both strains belonged to the genus with strain USC-21046 showing an 80.4 % genome similarity to NBRC 16497 and NBRC 100343, whereas USC-21048 strain showed an 83.6 % genome similarity to NBRC 100429. Both strains were delineated from their closely related relatives based on physiological (e.g. growth on sole carbon source) and chemotaxonomic (e.g. cellular fatty composition) differences. The digital DNA–DNA hybridization (dDDH) values between USC-21046 and USC-21048 and their closely related relatives were below the dDDH threshold value of ≤70 % used for the taxonomic classification of novel species status. The genome length of strains USC-21046 and USC-21048 were 6 878 863 and 7 066 978 bp, with G+C contents of 65.2 and 67.8 mol%, respectively. For the novel isolates, we propose the names sp. nov. with the type strain USC-21046 (=DSM 111727=NCCB 100867) and sp. nov. with the type strain USC-21048 (=DSM 111726=NCCB 100868).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Australia , beach , foam , foaming coastal marine waters , Nocardia , nocardiae , novel and Queensland
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-05-09
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Nocardia australiensis sp. nov. and Nocardia spumae sp. nov., isolated from sea foam in Queensland, Australia
Int J Syst Evol Microbiol 73, 005952 (2023); https://doi.org/10.1099/ijsem.0.005952
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