1887

Abstract

Strain HF14-78462 is an environmental bacterium found in clinical samples from an immunocompromized patient in 2014 at Hospital Universitari i Politècnic La Fe (Valencia, Spain). Phenotypically, strain HF14-78462 cells were Gram-stain-negative, aerobic, non-spore forming and non-motile small rods which formed mucous and whitish-translucent colonies when incubated at 20–36 °C. Phylogenetic analyses based on the 16S rRNA genes and the whole genomes of closest sequenced relatives confirmed that strain HF14-78462 is affiliated with the genus . The strain was oxidase, catalase and urease positive; but indole, lysine decarboxylase, ornithine decarboxylase and DNase negative, did not produce HS and was able to utilize a wide variety of carbon sources including acetamide, adonitol, amygdalin, -arabinose, citric acid, glucose, mannitol and melibiose. Unlike and , strain HF14-78462 failed to grow in thiosulphate-oxidizing media and had a narrower temperature growth range. Its genome was characterized by a size of 4.83 Mbp and a C+G content of 67.75 mol%. Major fatty acids were C 7, cyclo C and C, its polar acids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an aminophospholipid; while the ubiquinones were Q9 (1.8 %) and Q10 (98.2 %). Digital DNA–DNA hybridization values were 41 and 41.4 against and , respectively, while average nucleotide identity values were around 84 %. Phenotypic, average nucleotide identity and phylogenomic comparative studies suggest that strain HF14-78462 is a new representative of the genus and the name sp. nov. is proposed. The type strain is HF14-78462 (=CECT 30124=LMG 31874).

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2023-08-21
2024-07-25
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