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Abstract

We investigated a taxonomically novel group of the genus , which included five strains isolated from soil and water samples collected in preserved forest areas in Czechia between 2013 and 2021. The whole-genome sequences of the strains were 3.1–3.2 Mb in size, with G+C contents of 38.0–38.2 mol%. Core genome-based phylogenetic analysis showed that they formed a compact and deeply branched clade within the genus. The genomic average nucleotide identity based on /digital DNA–DNA hybridization values for the novel strains were 99.2–99.6 %/95.2–98.4 %, whereas those between the novel strains and the type strains of the known species reached <78 %/<24 %. The results of the genus-wide analysis of whole-cell MALDI-TOF mass spectra supported the sharp distinctness of the group. The five strains were non-glucose acidifying, nonhaemolytic, nonproteolytic and growing at 28 °C, but not at 32 °C; they assimilated acetate, benzoate, ethanol, -histidine, 4-hydroxybenzoate, -lactate and malonate but not 4-aminobutyrate, -aspartate or 2,3-butanediol; this phenotype is unique among the known species. We conclude that the five strains represent a novel environmental species, for which the name sp. nov. is proposed, with the type strain ANC 4999 (=CCM 9207=CCUG 75877=CNCTC 8124).

Funding
This study was supported by the:
  • Grantová Agentura České Republiky (Award 22-05373S)
    • Principle Award Recipient: AlexandrNemec
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/content/journal/ijsem/10.1099/ijsem.0.005383
2022-04-19
2024-07-25
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