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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 4

Genome-based, phenotypic and chemotaxonomic classification of strains: proposal of three novel species sp. nov., sp. nov. and sp. nov. No Access

Abstract

is one of the most important butyrate-producing bacteria in the human gut. Previous studies have suggested the presence of several phylogenetic groups, with differences at the species level, in the species, and a taxonomic re-evaluation is thus essential for further understanding of ecology of the important human symbiont. Here we examine the phenotypic, physiological, chemotaxonomic and phylogenomic characteristics of six strains (BCRC 81047=ATCC 27768, A2-165=JCM 31915, APC918/95b=JCM 39207, APC942/30−2=JCM 39208, APC924/119=JCM 39209 and APC922/41−1=JCM 39210) deposited in public culture collections with two reference strains of JCM 39212 and JCM 39211. sp. JCM 17207 isolated from caecum of broiler chicken was also included. Three strains of (BCRC 81047, JCM 39207 and JCM 39209) shared more than 96.6 % average nucleotide identity (ANI) and 69.6 % digital DNA–DNA hybridization (dDDH) values, indicating that the three strains are members of the same species. On the other hand, the remaining three strains of (JCM 31915, JCM 39208 and JCM 39210) were clearly separated from the above three strains based on the ANI and dDDH values. Rather, JCM 39208 showed ANI and dDDH values over the cut-off values of species discrimination (>70 % dDDH and >95–96 % ANI) with JCM 39211, whereas JCM 31915, JCM 39210 and JCM 17207 did not share dDDH and ANI values over the currently accepted cut-off values with any of the tested strains, including among them. Furthermore, the cellular fatty acid patterns of these strains were slightly different from other strains. Based on the collected data, JCM 31915, JCM 39210 and sp. JCM 17207 represent three novel species of the genus , for which the names sp. nov. (type strain JCM 31915=DSM 17677=A2-165), sp. nov. (type strain JCM 39210=DSM 107841=APC922/41-1) and sp. nov. (type strain JCM 17207=DSM 23680=ic1379) are proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Faecalibacterium duncaniae , Faecalibacterium gallinarum , Faecalibacterium hattorii , Faecalibacterium prausnitzii and Genome-based taxonomy
Funding
This study was supported by the:
  • Japan Society for the Promotion of Science (Award 20K05792)
    • Principle Award Recipient: AkihitoEndo
  • Japan Society for the Promotion of Science (Award 19H05679)
    • Principle Award Recipient: MoriyaOhkuma
  • Japan Society for the Promotion of Science (Award JP21J11100)
    • Principle Award Recipient: HirokiTanno
  • Japan Agency for Medical Research and Development (Award JP21ae0121035)
    • Principle Award Recipient: MitsuoSakamoto
© 2022 The Authors
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2022-04-13
2024-05-13
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Genome-based, phenotypic and chemotaxonomic classification of Faecalibacterium strains: proposal of three novel species Faecalibacterium duncaniae sp. nov., Faecalibacterium hattorii sp. nov. and Faecalibacterium gallinarum sp. nov.
Int J Syst Evol Microbiol 72, 005379 (2022); https://doi.org/10.1099/ijsem.0.005379
/content/journal/ijsem/10.1099/ijsem.0.005379
/content/journal/ijsem/10.1099/ijsem.0.005379
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