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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 4

gen. nov. sp. nov., a neutrophilic, microaerobic iron- and thiosulfate-oxidizing bacterium isolated from iron-rich wetland sediment No Access

Abstract

A neutrophilic iron-oxidizing bacterium, strain MIZ01, which was previously isolated from a wetland in Ibaraki, Japan, was taxonomically characterized in detail. Strain MIZ01 was a motile, curved-rod shaped, Gram-stain-negative bacterium. It was able to grow at 10–40 °C (optimally at 30–35 °C) and at pH 5.5–7.0 (optimally at pH 6.0). It grew microaerobically and chemolithoautotrophically using thiosulfate, in addition to ferrous iron, as the sole electron donor. Major cellular fatty acids of strain MIZ01 were C 7/C 6 and C. The complete genome sequence (2.74 Mbp) was determined, showing that its DNA G+C content was 60.0 mol%. Phylogenetic analyses indicated that strain MIZ01 belonged to the family , class , and was closely related to an isolate tentatively named ‘’ ES-1 (98.2 % of 16S rRNA gene sequence similarity). Based on its phenotypic and phylogenetic characteristics, we conclude that strain MIZ01 represents a new genus and species in the family for which we propose the name gen. nov., sp. nov. The type strain is strain MIZ01 (=JCM 39089=DSM 111897).

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  • Accepted:
  • Published Online:
Keyword(s): bacteria , chemolithoautotroph , iron oxidation , microaerophile , neutrophile and thiosulfate oxidation
Funding
This study was supported by the:
  • Japan Society for the Promotion of Science (Award 19H03310)
    • Principle Award Recipient: ShingoKato
  • Japan Society for the Promotion of Science (Award 19H05689)
    • Principle Award Recipient: MoriyaOhkuma
  • Japan Society for the Promotion of Science (Award 19H05679)
    • Principle Award Recipient: MoriyaOhkuma
© 2022 The Authors
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2022-04-27
2024-05-06
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Sideroxyarcus emersonii gen. nov. sp. nov., a neutrophilic, microaerobic iron- and thiosulfate-oxidizing bacterium isolated from iron-rich wetland sediment
Int J Syst Evol Microbiol 72, 005347 (2022); https://doi.org/10.1099/ijsem.0.005347
/content/journal/ijsem/10.1099/ijsem.0.005347
/content/journal/ijsem/10.1099/ijsem.0.005347
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