1887

Abstract

Two enterobacterial strains, designated YMB-R21 and YMB-R22, were isolated from larvae of mealworm L. and examined for their taxonomic characteristics. A 16S rRNA gene-based neighbour-joining tree showed that the two isolates formed two distinct sublineages within the family and were separated from other genera of the family. The isolates showed 16S rRNA gene sequence similarity of 98.9 % to each other and ≤96.5 % to members of the order . The phylogenomic analysis based on 92 singly-copy core genes showed that the two isolates belonged to the family and formed a distinct sublineage at a position located remotely from the genera of the family. The loosely associated members were the type strain of and members of the genus . Average nucleotide identity and digital DNA–DNA hybridization values showed that the isolates represented members of a novel species in the family . The values of amino acid identity between the two isolates and the closest relatives were 74.5–75.0 % with the type strain of and 74.5–74.8 % with the type strains of two species, while showed the amino acid identity values of 76.3–76.5 % with two species. Based on the results obtained here, we propose a new genus with the description of sp. nov. (type strain YMB-R21=KCTC 82597=CCM 9152 and strain YMB-R22=KCTC 82598=CCM 9153), with the transfer of Liu . 2016 to a new genus as comb. nov. (type strain SCU-B244=CGMCC 1.12772=DSM 28222=KCTC 42022).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.005329
2022-04-22
2024-07-23
Loading full text...

Full text loading...

References

  1. Adeolu M, Alnajar S, Naushad S, S Gupta R. Genome-based phylogeny and taxonomy of the “Enterobacteriales”: proposal for Enterobacterales ord. nov. divided into the families Enterobacteriaceae, Erwiniaceae fam. nov., Pectobacteriaceae fam. nov., Yersiniaceae fam. nov., Hafniaceae fam. nov., Morganellaceae fam. nov., and Budviciaceae fam. nov. Int J Syst Evol Microbiol 2016; 66:5575–5599 [View Article]
    [Google Scholar]
  2. Soutar CD, Stavrinides J. Phylogenetic analysis supporting the taxonomic revision of eight genera within the bacterial order Enterobacterales. Int J Syst Evol Microbiol 2020; 70:6524–6530 [View Article] [PubMed]
    [Google Scholar]
  3. Brandon AM, Gao S-H, Tian R, Ning D, Yang S-S et al. Biodegradation of polyethylene and plastic mixtures in mealworms (larvae of Tenebrio molitor) and effects on the gut microbiome. Environ Sci Technol 2018; 52:6526–6533 [View Article] [PubMed]
    [Google Scholar]
  4. Peng B-Y, Su Y, Chen Z, Chen J, Zhou X et al. Biodegradation of polystyrene by dark (Tenebrio obscurus) and yellow (Tenebrio molitor) mealworms (Coleoptera: tenebrionidae). Environ Sci Technol 2019; 53:5256–5265 [View Article]
    [Google Scholar]
  5. Lee SD, Kim IS, Choe H, Kim J-S. Acerihabitans arboris gen. nov., sp. nov., a new member of the family pectobacteriaceae isolated from sap drawn from acer acer pictum. Int J Syst Evol Microbiol 2021; 71:004674 [View Article]
    [Google Scholar]
  6. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article] [PubMed]
    [Google Scholar]
  7. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article] [PubMed]
    [Google Scholar]
  8. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Biol 1971; 20:406–416 [View Article]
    [Google Scholar]
  9. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article] [PubMed]
    [Google Scholar]
  10. Jukes TH, Cantor CR. Evolution of protein molecules. In Munro HN. eds Mammalian Protein Metabolism New York: Academic Press; 1969 pp 21–132
    [Google Scholar]
  11. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article] [PubMed]
    [Google Scholar]
  12. Na S-I, Kim YO, Yoon S-H, Ha S-M, Baek I et al. UBCG: Up-to-date bacterial core gene set and pipeline for phylogenomic tree reconstruction. J Microbiol 2018; 56:280–285 [View Article] [PubMed]
    [Google Scholar]
  13. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: A taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article] [PubMed]
    [Google Scholar]
  14. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article] [PubMed]
    [Google Scholar]
  15. Rodriguez-R LM, Konstantinidis KT. Bypassing cultivation to identify bacterial species. Microbe Magazine 2014; 9:111–118 [View Article]
    [Google Scholar]
  16. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 2009; 106:19126–19131 [View Article] [PubMed]
    [Google Scholar]
  17. Goris J, Konstantinidis KT, Klappenbach JA, Coenye T, Vandamme P et al. DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 2007; 57:81–91 [View Article] [PubMed]
    [Google Scholar]
  18. Wirth JS, Whitman WB. Phylogenomic analyses of a clade within the Roseobacter group suggest taxonomic reassignments of species of the genera Aestuariivita, Citreicella, Loktanella, Nautella, Pelagibaca, Ruegeria, Thalassobius, Thiobacimonas and Tropicibacter, and the proposal of six novel genera. Int J Syst Evol Microbiol 2018; 68:2393–2411 [View Article] [PubMed]
    [Google Scholar]
  19. Nicholson AC, Gulvik CA, Whitney AM, Humrighouse BW, Bell ME et al. Division of the genus Chryseobacterium: Observation of discontinuities in amino acid identity values, a possible consequence of major extinction events, guides transfer of nine species to the genus Epilithonimonas, eleven species to the genus Kaistella, and three species to the genus Halpernia gen. nov., with description of Kaistella daneshvariae sp. nov. and Epilithonimonas vandammei sp. nov. derived from clinical specimens. Int J Syst Evol Microbiol 2020; 70:4432–4450 [View Article]
    [Google Scholar]
  20. Liu B, Luo J, Li W, Long X-F, Zhang Y-Q et al. Erwinia teleogrylli sp. nov., a bacterial isolate associated with a Chinese cricket. PLoS One 2016; 11:e0146596 [View Article] [PubMed]
    [Google Scholar]
  21. Kroppenstedt RM. Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Goodfellow M, Minnikin DE. eds Chemical Methods in Bacterial Systematics London: Academic Press; 1985 pp 173–199
    [Google Scholar]
  22. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  23. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M. Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 1977; 27:104–117 [View Article]
    [Google Scholar]
  24. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T et al. The RAST Server: Rapid Annotations Using Subsystems Technology. BMC Genomics 2008; 9:75 [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.005329
Loading
/content/journal/ijsem/10.1099/ijsem.0.005329
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error