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Abstract

A Gram-stain-positive, aerobic actinobacterial strain designated MMS17-BM035 isolated from mountain soil around a decaying tree was subjected to taxonomic characterization. The isolate developed extensively branched substrate mycelia and white aerial hyphae on International Project 2 agar. Strain MMS17-BM035 grew at 15–34 °C (optimum, 30 °C), at pH 5.0–8.0 (optimum, pH 7.0) and in the presence of 0–6 % NaCl (optimum, 0 %). Analysis of 16S rRNA gene sequences indicated that MMS17-BM035 fell into a phylogenetic cluster belonging to the genus . MMS17-BM035 shared the highest sequence similarity of 99.45 % with JBL-20, and no higher than 98.7 % with other species of . Based on the orthologous average nucleotide identity, MMS17-BM035 was again mostly related to JBL-20 with 84.14 % identity, and less than 80 % with other species. The digital DNA–DNA hybridization analysis also indicated low levels of relatedness with related species, as the highest value was observed with JBL-20 (28.8 %). The major fatty acids of the strain were anteiso-C, a summed feature (consisting of C ω7/C ω6), iso-C, C and C. The major respiratory quinones were MK-9(H) and MK-9(H). The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositolmannoside. The major cell-wall diamino acid was -diaminopimelic acid, and the characteristic whole-cell sugars were glucose and ribose. The DNA G+C content was 72.1 mol%. Strain MMS17-BM035 exhibited antimicrobial activity against several Gram-positive bacteria and yeasts. Based on both phenotypic and phylogenetic evidences, strain MMS17-BM035 should be classified as representing a novel species, for which the name sp. nov. (type strain=MMS17-BM035=KCTC 49544=JCM 34528) is proposed.

Funding
This study was supported by the:
  • National Research Foundation of Korea
    • Principle Award Recipient: SeungBum Kim
  • National Institute of Biological Resources
    • Principle Award Recipient: SeungBum Kim
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2022-04-20
2022-07-06
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