1887

Abstract

Four halophilic archaeal strains, designated HD8-83, LYG-36, DLLS-82 and RC-68, were isolated from the salted brown alga of three different origins (Dalian, Lianyungang, Dalian and Rongcheng) in PR China. All strains had pleomorphic rod cells that were motile, lysed in distilled water, stained Gram-negative, and formed red-pigmented colonies on agar plate (except for DLLS-82, which formed white colonies). Based on phylogenetic analyses of the 16S rRNA genes, strain HD8-83 was closely related to HD8-51 (97.9 % similarity), strain LYG-36 and DLLS-82 to TBN4 (94.4 % and 94.7 % similarities, respectively), and strain RC-68 to YJ-37-H (96.9 % similarity). Results of phylogenetic analyses based on genes and 728 concatenated single-copy orthologous clusters also showed that these strains formed three different branches and clustered tightly with the members. The average nucleotide identity, average amino acid identity and DNA–DNA hybridization values between strains LYG-36 and DLLS-82 were 98.9, 98 and 92.4%, showing that they were different strains of the same species. While those values between the isolates and other members were below 84.7, 82.9 and 28.9 %, respectively. Based on the phenotypic, chemotaxonomic and phylogenetic properties, strains HD8-83, LYG-36, DLLS-82 and RC-68 represent three novel species of the genus for which the names sp. nov. (type strain: HD8-83=CGMCC 1.15334=JCM 31110), sp. nov. (type strain: LYG-36=CGMCC 1.13606=JCM 32952; reference strain: DLLS-82=CGMCC 1.13604=JCM 32951) and sp. nov. (type strain: RC-68=CGMCC 1.13609=JCM 32953) are proposed.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31770005)
    • Principle Award Recipient: Heng-LinCui
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2022-04-07
2022-05-18
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