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Volume 72, Issue 4

sp. nov., a bacterial pathogen causing onion bulb rot No Access

Abstract

A Gram-stain-negative, aerobic and non-spore-forming bacterial strain, designated 20TX0172, was isolated from a rotting onion bulb in Texas, USA. The results of phylogenetic analysis based on the 16S rRNA sequence indicated that the novel strain represented a member of the genus and had the greatest sequence similarities with 520-20 (99.3 %), CFBP 2431 (99.2 %), and 11K1 (99.2 %) but the 16S rRNA phylogenetic tree displayed a monophyletic clade with CFBP 5447. In the phylogenetic trees based on sequences of four housekeeping genes (, , and ), the novel strain formed a separate branch, indicating that the strain was distinct phylogenetically from known species of the genus . The genome-sequence-derived average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between the novel isolate and DSM 16733 were 86.7 and 32.7 %, respectively. These values were below the accepted species cutoff threshold of 96 % ANI and 70 % dDDH, affirming that the strain represented a novel species. The genome size of the novel species was 5.98 Mbp with a DNA G+C content of 60.8 mol%. On the basis of phenotypic and genotypic characteristics, strain 20TX0172 represents a novel species of the genus . The name sp. nov. is proposed. The type strain is 20TX0172 (=NCIMB 15426=CIP 112022).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA , average nucleotide identity , digital DNA-DNA hybridization , fatty acid methyl ester , novel species , onion , Pseudomonas and whole genome sequence
Funding
This study was supported by the:
  • Agricultural Research Service (Award 2019-51181-30013)
    • Principle Award Recipient: SubasMalla
© 2022 The Authors
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2022-04-20
2024-05-14
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Pseudomonas uvaldensis sp. nov., a bacterial pathogen causing onion bulb rot
Int J Syst Evol Microbiol 72, 005311 (2022); https://doi.org/10.1099/ijsem.0.005311
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