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Volume 71, Issue 10

sp. nov., a new member of the complex, isolated from human clinical specimens No Access

Abstract

and are the newest members of the complex. The number of clinical reports attributed to these new complex members is limited. In a retrospective clinical laboratory study conducted over a 4-month period investigating the prevalence of and , a total of 43 isolates were selected. Phylogeny based on core-gene multilocus sequence typing (MLST) analysis confirmed that 37 were but a genetically distinct clade of six isolates was identified. Digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) analyses further supported the classification of these six isolates as a separate species. When compared to complex reference genomes, the ANI values were ≤94 % and the dDDH values were <53 %. Based on the seven-gene MLST scheme, the six isolates belong to five novel allelic profiles (ST6105, ST6106, ST6107, ST6108 and ST109). Their clinical infection features were similar to . Skin and soft tissue infections presented in four out of the six cases. Routine clinical diagnostic identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and biochemical profiling does not differentiate these new members from the rest of the complex. Genotypic analysis suggests that the six isolates belong to a novel species, sp. nov. with isolate SS21 (=DSM 111408=NCTC14419) designated as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): clinical features , phenotypic typing , Staphylococcus argenteus , Staphylococcus aureus complex and whole-genome-sequencing
© 2021 The Authors
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2021-10-26
2024-05-13
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Staphylococcus singaporensis sp. nov., a new member of the Staphylococcus aureus complex, isolated from human clinical specimens
Int J Syst Evol Microbiol 71, 005067 (2021); https://doi.org/10.1099/ijsem.0.005067
/content/journal/ijsem/10.1099/ijsem.0.005067
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