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Volume 71, Issue 10

sp. nov., isolated from an oil production mixture in Yumen Oilfield, and emended description of No Access

Abstract

A pink, ovoid-shaped, Gram-stain-negative, strictly aerobic and motile bacterial strain, designated ROY-5-3, was isolated from an oil production mixture from Yumen Oilfield in PR China. The strain grew at 4–42 °C (optimum, 30 °C), at pH 5–10 (optimum, 7) and with 0–5 % (w/v) NaCl (optimum, 0%). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that ROY-5-3 belongs to the genus and shared the highest pairwise similarities with CW67 (98.1%), BU-1 (97.8%), K-20 (97.7%) and HS-69 (97.3%). The average nucleotide identity and digital DNA–DNA hybridization values between ROY-5-3 and other related type strains of species were less than 84.08 and 28.60 %, respectively, both below the species delineation threshold. Pan-genomic analysis showed that the novel isolate ROY-5-3 shared 3265 core gene families with the four closely related type strains in , and the number of strain-specific gene families was 513. The major fatty acids were identified as summed feature 8 (C 6/C 7), summed feature 3 (C 6/C 7) and C. Strain ROY-5-3 contained Q-10 as the main ubiquinone and the genomic DNA G+C content was 69.8 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. Based on the phylogenetic, morphological, physiological, chemotaxonomic and genome analyses, strain ROY-5-3 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is ROY-5-3 (=CGMCC 1.13459 =KCTC 82484).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genome , oil production mixture , polyphasic taxonomy and Roseomonas oleicola sp. nov
Funding
This study was supported by the:
  • biological resources programme of chinese academy of sciences-the capacity-building project (Award KFJ-BRP-017-25)
    • Principle Award Recipient: ManCai
  • national key r & d program of china (Award 2018YFA0902100)
    • Principle Award Recipient: XiaoleiWu
© 2021 The Authors
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2021-10-28
2024-04-29
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Roseomonas oleicola sp. nov., isolated from an oil production mixture in Yumen Oilfield, and emended description of Roseomonas frigidaquae
Int J Syst Evol Microbiol 71, 005064 (2021); https://doi.org/10.1099/ijsem.0.005064
/content/journal/ijsem/10.1099/ijsem.0.005064
/content/journal/ijsem/10.1099/ijsem.0.005064
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