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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 10

Description of antibiotic-producing novel bacteria sp. nov. and sp. nov. No Access

Abstract

Two white colony-forming, Gram-stain-negative, non-sporulating and motile bacteria, designated G-4-1-8 and RP-4-7, were isolated from forest soil and Arctic soil, respectively. Both strains showed antimicrobial activity against Gram-negative pathogens ( and ) and could grow at a pH range of pH 4.0–11.0 (optimum, pH 7.0–9.0). Phylogenetic analyses based on their 16S rRNA gene sequences indicated that strains G-4-1-8 and RP-4-7 formed a lineage within the family and were clustered as members of the genus . Strain G-4-1-8 showed the highest 16S rRNA sequence similarity to JC2948 (98.1 %), while strain RP-4-7 showed the highest similarity to DNBP6-1 (98.8 %). The only respiratory quinone in both strains was ubiquinone Q-8. Their principal cellular fatty acids were C, cyclo-C, summed feature 3 (iso-C 2-OH and/or C 7) and summed feature 8 (C 7 and/or C 6). Their major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unidentified aminophospholipid. The DNA G+C content of strains G-4-1-8 and RP-4-7 were 63.7 and 61.3 mol%, respectively, while their genome lengths were 7.44 and 9.67 Mb, respectively. The genomes of both strains showed at least 12 putative biosynthetic gene clusters. The average nucleotide identity and DNA–DNA hybridization relatedness values between both strains and most closely related species were below the species threshold values. Based on a polyphasic study, these isolated strains represent novel species belonging to the genus , for which the names sp. nov. (G-4-1-8= KACC 21617=NBRC 114603) and sp. nov. (RP-4-7=KACC 21621=NBRC 114605) are proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antimicrobial agents , antimicrobial resistance , Arctic soil , forest soil and psychrotolerant
Funding
This study was supported by the:
  • national research foundation (kr) (Award 2019R1F1A1058501)
    • Principle Award Recipient: JaisooKim
© 2021 The Authors
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2021-10-25
2024-04-29
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Description of antibiotic-producing novel bacteria Paraburkholderia antibiotica sp. nov. and Paraburkholderia polaris sp. nov.
Int J Syst Evol Microbiol 71, 005060 (2021); https://doi.org/10.1099/ijsem.0.005060
/content/journal/ijsem/10.1099/ijsem.0.005060
/content/journal/ijsem/10.1099/ijsem.0.005060
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