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Volume 71, Issue 10

sp. nov., an acetic acid bacterium isolated from fermented mangosteen peel in Thailand No Access

Abstract

Two isolates, MS16-SU-2 and MS18-SU-3, obtained from fermented mangosteen peel in vinegar were suggested to constitute a new species assignable to the genus based on the results of 16S rRNA gene sequencing. The two isolates showed the highest sequence similarity (98.58%) to NBRC 16470 and LMG 23690. However, the calculated similarity values were lower than the threshold for species demarcation. The phylogenetic analysis showed that the branches of the two isolates were separated from other species, and the two isolates constituted a new species in the genus . The genomic DNA of isolate MS16-SU-2 was sequenced. The assembled genome of the isolate was analysed, and the results showed that the highest average nucleotide identity value of 75.9 % was with JCM 25143 and the highest digital DNA–DNA hybridization value of 25.1 % was with LMG 1636, which were lower than the cutoff values for species delineation. The phylogenetic tree based on the genome sequences showed that the lineage of isolate MS16-SU-2 was most closely related to JCM 25143 and TBRC 1719, but separated from the branches of these two species. In addition, the two isolates could be distinguished from the type strains of closely related species by their phenotypic characteristics and MALDI-TOF profiles. Therefore, the two isolates, MS16-SU-2 (=TBRC 12339=LMG 32243) and MS18-SU-3 (=TBRC 12305), can be assigned to an independent species within the genus , and the name of sp. nov. is proposed for the two isolates.

  • Received:
  • Accepted:
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Keyword(s): acetic acid bacteria , Acetobacter garciniae sp. nov. , mangosteen peel and Thailand
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2021-10-18
2024-04-29
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Acetobacter garciniae sp. nov., an acetic acid bacterium isolated from fermented mangosteen peel in Thailand
Int J Syst Evol Microbiol 71, 005052 (2021); https://doi.org/10.1099/ijsem.0.005052
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