1887

Abstract

Through this study, we established the taxonomic status of seven strains belonging to the genus (A477-S1-J17, A398-S21-F17, A535-S3-A17, A411-S4-F17, A113-S21-F16, FL63-S17 and FL60-S17) collected from four different river streams and an artificial lake in south-east France between 2016 and 2017. Ecological surveys in rivers and lakes pointed out different repartition of strains belonging to this clade compared to the closest species, . The main phenotypic difference observed between these strains and the type strain was strongly impaired growth with rhamnose as the sole carbon source. This correlates with three different forms of pseudogenization of the -rhamnose/proton symporter gene in the genomes of strains belonging to this clade. Phylogenetic analysis using gene sequences and multi locus sequence analysis of the core genome showed that these strains formed a distinct clade within the genus closely related to Digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values showed a clear discontinuity between the new clade and . However, the calculated values are potentially consistent with either splitting or merging of this new clade with . In support of the split, ANI coverages were higher within this new clade than between this new clade and . The split is also consistent with the range of observed ANI or dDDH values that currently separate several accepted species within the genus . On the basis of these data,strains A477-S1-J17, A398-S21-F17, A535-S3-A17, A411-S4-F17, A113-S21-F16, FL63-S17 and FL60-S17 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A477-S1-J17 (=CFBP 8805=LMG 32181).

Funding
This study was supported by the:
  • Centre National de la Recherche Scientifique (Award EC2COBiohefect/Ecodyn/Dril/MicrobienCARTOBACTER)
    • Principle Award Recipient: Marie-AnneBarny
  • Agence Nationale de la Recherche (Award ANR-17-CE32-0004)
    • Principle Award Recipient: Marie-AnneBarny
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