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Volume 71, Issue 10

sp. nov., a novel endophytic bacterium isolated from bark No Access

Abstract

A Gram-stain-negative, aerobic, short-rod-shaped bacterium, designated strain CBS1P-1, was isolated from a surface-sterilized bark of . Growth of strain CBS1P-1 was observed with between 0 and 12.0 % (w/v) NaCl (optimally with 5.0 %) and at between pH 6.0–9.0. It grew at temperatures between 25–37 °C (optimum, 30 °C). Chemotaxonomic analysis showed that ubiquinone-10 was the respiratory quinone. The lipids comprised diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid, an unidentified phospholipid and an unidentified aminolipid. The major fatty acids of strain CBS1P-1 were C 7, C and C cyclo 8. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CBS1P-1 was most related to CGMCC 1.12662 with a sequence similarity of 96.5 %. The average nucleotide identity and digital DNA–DNA hybridization values between strain CBS1P-1 and 1.12662 were 77.5 and 21.1 %, respectively. The G+C content of the genomic DNA was 67.3 mol%. Based on phylogenetic, chemotaxonomic and phenotypic data, strain CBS1P-1 is considered to represent a novel species of the genus , for which the name is proposed. The type strain is CBS1P-1 (=KCTC 62836=CGMCC 1.13743).

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  • Accepted:
  • Published Online:
Keyword(s): Aegiceras corniculatum , endophytic bacterium , novel species and Pseudooceanicola endophyticus
Funding
This study was supported by the:
  • Guizhou Provincial Science and Technology Foundation (Award Qian Ke He Jichu [2019]1347)
    • Principle Award Recipient: LiTuo
  • the National Natural Sciences Foundation of China (Award 81960642)
    • Principle Award Recipient: LiTuo
© 2021 The Authors
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2021-10-01
2024-04-29
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Pseudooceanicola endophyticus sp. nov., a novel endophytic bacterium isolated from bark Aegiceras corniculatum
Int J Syst Evol Microbiol 71, 005036 (2021); https://doi.org/10.1099/ijsem.0.005036
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