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Volume 71, Issue 10

sp. nov., a novel thermophilic bacterium isolated from the Karvachar geothermal spring in Nagorno-Karabakh Open Access

Abstract

Twelve thermophilic strains were isolated from sediment and water samples from a Karvachar hot spring located in the northern part of Nagorno-Karabakh. Based on phenotypic, chemotaxonomic and phylogenetic characteristics, one of the isolates, designated strain K1, was studied in detail. The cells are straight, motile rods that are 0.2–0.4×2.3–7.2 µm in size. The strain is a Gram-stain-positive, moderately thermophilic facultative anaerobe with an optimum growth temperature of 60–65 °C and a growth temperature range of 45–70 °C. Growth of strain K1 was observed at pH 6–11 (optimum, pH 8–9) and was inhibited in the presence of NaCl concentrations above 2.5 % (optimum, 1–1.5 %). The isolate could utilize a wide variety of carbon sources, including -arabinose, -ribose, -galactose, -fructose, -mannitol, maltose, aesculin, melibiose, sucrose, trehalose, raffinose, amidone, glycogen, turanose, -lyxose, -tagatose, potassium gluconate and 2-keto-gluconate. The strain was able to hydrolyse starch, casein and gelatin, was positive for oxidase and catalase, and reduced nitrate to nitrite, but was negative for HS production. Production of urease and indole was not observed. The major cellular fatty acids were C iso, C and C iso (52.5, 13.6 and 19.6 % of total fatty acids, respectively). Strain K1 shares >99 % 16S rRNA sequence similarity and a genomic average nucleotide identity value of 94.5 % with its closest relative, DSM 2641, suggesting that it represents a separate and novel species, for which the name sp. nov. is proposed. The type strain of is K1 (=DSM 106524=KCTC 15807).

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Keyword(s): Anoxybacillus , geothermal spring , next-generation sequencing , thermophiles and whole-genome sequencing
Funding
This study was supported by the:
  • direktoratet for internasjonalisering og kvalitetsutvikling i høgare utdanning (Award CPEA-LT-2016/10095 and CPEA-LT-2017/10061)
    • Principle Award Recipient: Nils-KåreBirkeland
  • state committee of science (Award 15T-F399 and 18T-1F261)
    • Principle Award Recipient: HovikPanosyan
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2021-10-01
2024-04-27
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Anoxybacillus karvacharensis sp. nov., a novel thermophilic bacterium isolated from the Karvachar geothermal spring in Nagorno-Karabakh
Int J Syst Evol Microbiol 71, 005035 (2021); https://doi.org/10.1099/ijsem.0.005035
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