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Volume 71, Issue 10

sp. nov., of the family , isolated from marine sediment No Access

Abstract

A Gram-stain-negative, ovoid or rod-shaped, non-flagellated, motile-by-gliding and aerobic bacteria, designated S10-8, was isolated from marine sediment of the Yellow Sea. Colonies of strain S10-8 had a pink-red pigmentation and its cells were approximately 0.5–0.8 μm×1.0–2.5 μm in size. Growth occurred at 10–45 °C (optimally at 33–37 °C), in the presence of 0–12.0 % NaCl (optimally at 2.0–5.0 %, w/v) and at pH 5.0–8.5 (optimally at pH 7.0–7.5). Phylogenetic analysis of the 16S rRNA gene indicated that strain S10-8 is a member of the genus within the family , and the 16S rRNA gene sequence similarity of strain S10-8 to its closest relative KCTC 12367 was 96.9 %. Strain S10-8 contained MK-7 as the predominant menaquinone and summed feature 4 (iso-C I and/or anteiso-C B) and iso-C as the major fatty acids. The major polar lipids were phosphatidylethanolamine, an unidentified aminophospholipid and an unidentified lipid. The size of the draft genome was 4 623 791 bp and the G+C content was 53.5 mol%. There were low DNA-DNA hybridization values (<48.3±5.2 %) and average nucleotide identity values (<86.5 %) between strain S10-8 and the most closely related recognized species. Therefore, we propose a novel species in the genus to accommodate the novel isolate: sp. nov. (type strain S10-8=KCTC 42769=ACCC 19859).

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  • Accepted:
  • Published Online:
Keyword(s): Hymenobacteraceae , marine sediment and Pontibacter flavimaris
Funding
This study was supported by the:
  • National Basic Research Program of China (973 Program) (Award 2017YFD0201401)
    • Principle Award Recipient: GuishanZhang
  • National Natural Science Foundation of China (Award NSFC No. 31670113)
    • Principle Award Recipient: GuishanZhang
© 2021 The Authors
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2021-10-11
2024-05-06
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Pontibacter flavimaris sp. nov., of the family Hymenobacteraceae, isolated from marine sediment
Int J Syst Evol Microbiol 71, 004958 (2021); https://doi.org/10.1099/ijsem.0.004958
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