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Abstract

A bacterial strain designated RZ02 was isolated from surface seawater collected from the Yellow Sea in PR China and characterized by polyphasic taxonomy. Cells of strain RZ02 were Gram-stain-negative, aerobic, non-motile, catalase- and oxidase-positive rods forming ochre-pigmented colonies. Growth occurred at 7–36 °C (optimum, 30 °C), at pH 6.0–9.0 (optimum, pH 7.0) and with 1–5 % (optimum, 2 %) NaCl. The major cellular fatty acids (>10 %) of strain RZ02 were summed feature 8 (C 6 and/or C 7), summed feature 3 (C 7 and/or C 6) and C. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol and sphingoglycolipid. The genome size of strain RZ02 was 2.79 Mbp with a G+C content of 55.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RZ02 was mostly related to SW-109 and HDW-31 (97.3 and 97.1% sequence similarity, respectively), and formed a phyletic lineage with members of the genus . The phylogenetic analysis based on the up-to-date bacterial core gene sequences confirmed that strain RZ02 clustered within the genus . The average nucleotide identity and DNA–DNA hybridization values between strain RZ02 and SW-109 and HDW-31 were 72.8 and 72.9 % and 18.7 and 18.5%, respectively. Based on these evidences, strain RZ02 is proposed to represent a novel species of the genus under the name sp. nov. The type strain is RZ02 (=KCTC 62828=MCCC 1K04521). In addition, based on the results of whole genome analyses, proposals of gen. nov., comb. nov. and comb. nov. are also included.

Funding
This study was supported by the:
  • Young Scientists Fund (Award 81801982)
    • Principle Award Recipient: AngLiu
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2021-04-22
2021-05-17
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