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Volume 71, Issue 4

sp. nov., isolated from a freshwater stream Free

Abstract

A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated as IMCC34836, was isolated from a freshwater stream. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain IMCC34836 was most closely related to HW001 (of the family ) with 95.6 % sequence similarity and formed a robust clade with HW001. The draft genome sequence of strain IMCC34836 was 4.4 Mbp in size with 59.1 mol% DNA G+C content. Average nucleotide identity and digital DNA–DNA hybridization values between strain IMCC34836 and HW001 were 71.2 and 22.0 %, respectively, indicating that the new strain represents a novel species. The strain contained iso-C, summed feature 3 (C 6 and/or C 7) and summed feature 9 (iso-C 9 and/or C 10-methyl) as the major fatty acids and harboured phosphatidylethanolamine, two unidentified aminophospholipids and three unidentified lipids as major polar lipids. The isoprenoid quinone detected in the strain was ubiquinone-8. Based on the phylogenetic and phenotypic characteristics, strain IMCC34836 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IMCC34836 (=KACC 21755=NBRC 114416).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): freshwater , genome , Permianibacter , Permianibacter fluminis and taxonomy
Funding
This study was supported by the:
  • Inha University
    • Principle Award Recipient: Jang-CheonCho
  • Ministry of Environment in Korea
    • Principle Award Recipient: Jang-CheonCho
© 2021 The Authors
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2021-04-21
2024-05-13
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Permianibacter fluminis sp. nov., isolated from a freshwater stream
Int J Syst Evol Microbiol 71, 004771 (2021); https://doi.org/10.1099/ijsem.0.004771
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