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Volume 71, Issue 4

sp. nov., isolated from the pod of soybean, Free

Abstract

Soybean pods, separated and enclosed from the outside environment, are considered a suitable place to find new microbes. A Gram-stain-negative, aerobic bacterium, bacterial strain (YB22) was isolated from the pod of (soybean) collected from a rural area in Republic of Korea and characterized by using polyphasic taxonomy. Cells of the strain were rod-shaped (approximately 0.4–0.6 µm wide and 4.0–5.0 µm long), non-flagellated and formed silver-yellow colonies. Cells grew at 25–35 °C (optimum, 28–30 °C), at pH 5.0–9.0 (optimum, pH 7.0) and with 0–2.0% NaCl (optimum, 0 % NaCl). 16S rRNA gene sequencing showed that strain YB22 was phylogenetically closest to the genus , and showed highest similarities to G4070 (96.7 %), ATCC 13253 (96.7 %), DSM 14571 (96.6 %), G0146 (96.5 %), G4122 (96.4 %) and R26 (96.2 %). Average amino acid identity values between strain YB22 and other taxa in the genus were all above the threshold range of genus determination. Average nucleotide identity and digital DNA–DNA hybridization values between strain YB22 and other phylogenetic relatives were all found to be below the threshold range for species determination. The respiratory quinone of strain YB22 was menaquinone 6 (MK-6) and the predominant cellular fatty acids were iso-C (47.8 %) and iso-C 3-OH (18.5 %). The major polar lipids were phosphatidylethanolamine, four unidentified aminolipids and three unidentified polar lipids. The phylogenetic analysis and physiological and biochemical data showed that strain YB22 should represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain for this novel species is YB22 (=KCCM 43263=JCM 32097).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): average amino acid identity , average nucleotide identity , Elizabethkingia , polyphasic taxonomy , soybean and whole genome
Funding
This study was supported by the:
  • Ministry of Education (Award 2020R1A6A1A03043283)
    • Principle Award Recipient: SangJoonMo
© 2021 The Authors
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2021-04-09
2024-04-26
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Elizabethkingia argenteiflava sp. nov., isolated from the pod of soybean, Glycine max
Int J Syst Evol Microbiol 71, 004767 (2021); https://doi.org/10.1099/ijsem.0.004767
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