1887

Abstract

We isolated two new soil bacteria: ONC3 (from garden soil in NC, USA; LMG 31738=NRRL B-65553) and M1 (from farmed soil in MI, USA; NRRL B-65551=ATCC TSD-197=LMG 31739) and characterized their metabolic phenotype based on Biolog, MALDI-TOF MS and fatty acid analyses, and compared 16S rRNA and whole genome sequences to other members of the after sequencing on an Illumina Nextera platform. Based on the results of 16S rRNA sequence analysis, ONC3 shows the highest sequence similarity to J18 (97.8 %), J11 (97.7 %) and J9 (97.3 %). Strain M1 is most closely related to TSA40, K-1-15 and TSA66 (sequence identity of 98.2, 98.0 and 97.8 %, respectively). The whole genome of ONC3 has an assembled size of 5.62 Mbp, a G+C content of 63.8 mol% and contains 5104 protein-coding sequences, 56 tRNA genes and two rRNA operons. The genome of M1 has a length of 4.71 MBp, a G+C content of 63.81 mol% and includes 4967 protein-coding genes, two rRNA operons and 44 tRNA genes. Whole genome comparisons identified sp. WG5 with a 79.3 % average nucleotide identity (ANI) and 22.6 % digital DNA–DNA hybridization (dDDH), and sp. UBA11196 with 78.2 % average amino acid identity (AAI) as the most closely related species to ONC3. M1 is most closely related to TSA66 with an ANI of 80.27 %, or TSA40 with a dDDH of 22.3 %. The application of community-accepted standards such as <98.7 % in 16S sequence similarity and <95–96 % ANI or 70 % DDH support the classification of ONC3 and M1 as novel species within the .

Funding
This study was supported by the:
  • Agricultural Experiment Station, South Dakota State University
    • Principle Award Recipient: BückingHeike
  • Novozymes North America
    • Principle Award Recipient: BückingHeike
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2021-06-19
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