Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 71, Issue 1

sp. nov., isolated from aquatic environments in the northern PR China Free

Abstract

Four strains (km711, km714, km542 and km524), representing a novel species, were isolated from aquatic environments in northern PR China. Cells were Gram-stain-negative, rod-shaped, microaerobic, motile and growth depended on -cysteine. They grew at 25‒42 °C (optimum, 35‒37 °C) and could tolerate up to 1.5 % (w/v) NaCl (optimum, 0.5 %). The major fatty acids (>5 %) of the type strain km711 were C anteiso, C anteiso, iso-C and C 7 and/or iso-C 2OH. The pairwise comparison values were <96.1 % for 16S rRNA gene sequences, 23.3‒28.7 % interspecies variation for gene sequences, <93.6 % average nucleotide identity and <72.8 % average amino acid identity between these four strains and related type strains within the genus . The phylogenetic tree based on the four concatenated genes (16S rRNA, , and ) and protein-concatamer tree based on concatenation of 21 protein markers both revealed that these four strains formed a separate phylogenetic branch cluster within the genus . The results of phenotypic and genotypic features suggest that these four strains represent a novel species of the genus , for which the name sp. nov. is proposed (type strain km711=KCTC 15655=NBRC 113219).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): aquatic environments , Legionella septentrionalis sp. nov. and polyphasic taxonomy
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award No. 31870001)
    • Principle Award Recipient: Xiao-YongZhan
  • Science and Technology Planning Project of Guangdong Province (Award 2017A020215068)
    • Principle Award Recipient: Liang-HuiLi
© 2021 The Authors

Erratum

An erratum has been published for this content:
Corrigendum: sp. nov., isolated from aquatic environments in the northern PR China
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004592
2020-12-07
2025-01-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/71/1/ijsem004592.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.004592&mimeType=html&fmt=ahah

References

  1. Washington CW. Family I. Legionellaceae. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed, vol. 2, The Proteobacteria New York: Springer; 2005 pp 237–263
     [Google Scholar]
  2. Edelstein PH, Lück C. Legionella. In Jorgensen JH, Pfaller MA. (editors) Manual of Clinical Microbiology 1, 11th ed. Washington, DC: ASM; 2015 pp 916–933
     [Google Scholar]
  3. Wu H-Y, Yan H, Zheng M-L, Sun M-M, Wang Q et al. Legionella qingyii sp. nov., isolated from water samples in China. Int J Syst Evol Microbiol 2019; 69:2017–2022 [View Article][PubMed]
     [Google Scholar]
  4. Zhan X-Y, Hu C-H, Zhu Q-Y. Targeting single-nucleotide polymorphisms in the 16S rRNA gene to detect and differentiate Legionella pneumophila and non-Legionella pneumophila species. Arch Microbiol 2016; 198:591–594 [View Article][PubMed]
     [Google Scholar]
  5. Ratcliff RM, Lanser JA, Manning PA, Heuzenroeder MW. Sequence-based classification scheme for the genus Legionella targeting the mip gene. J Clin Microbiol 1998; 36:1560–1567 [View Article][PubMed]
     [Google Scholar]
  6. Guan W, Xu Y, Chen D-L, Xu J-N, Tian Y et al. Application of multilocus sequence analysis (MLSA) for accurate identification of Legionella spp. Isolated from municipal fountains in Chengdu, China, based on 16S rRNA, mip, and rpoB genes. J Microbiol 2012; 50:127–136 [View Article][PubMed]
     [Google Scholar]
  7. Rubin CJ, Thollesson M, Kirsebom LA, Herrmann B. Phylogenetic relationships and species differentiation of 39 Legionella species by sequence determination of the RNase P RNA gene rnpB . Int J Syst Evol Microb 2049; iol 2005:2039
     [Google Scholar]
  8. Johnson M, Zaretskaya I, Raytselis Y, Merezhuk Y, McGinnis S et al. NCBI blast: a better web interface. Nucleic Acids Res 2008; 36:W5–W9 [View Article][PubMed]
     [Google Scholar]
  9. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
     [Google Scholar]
  10. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
     [Google Scholar]
  11. Yarza P, Yilmaz P, Pruesse E, Glöckner FO, Ludwig W et al. Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nat Rev Microbiol 2014; 12:635–645 [View Article][PubMed]
     [Google Scholar]
  12. Abbas MM, Malluhi QM, Balakrishnan P. Assessment of de novo assemblers for draft genomes: a case study with fungal genomes. BMC Genomics 2014; 15 Suppl 9:S10 [View Article][PubMed]
     [Google Scholar]
  13. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW et al. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 2015; 25:1043–1055 [View Article][PubMed]
     [Google Scholar]
  14. Wu M, Scott AJ. Phylogenomic analysis of bacterial and archaeal sequences with AMPHORA2. Bioinformatics 2012; 28:1033–1034 [View Article][PubMed]
     [Google Scholar]
  15. Edgar RC. Muscle: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004; 32:1792–1797 [View Article][PubMed]
     [Google Scholar]
  16. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014; 30:1312–1313 [View Article][PubMed]
     [Google Scholar]
  17. Letunic I, Bork P. Interactive tree of life (iTOL) V3: an online tool for the display and annotation of phylogenetic and other trees. Nucleic Acids Res 2016; 44:W242–W245 [View Article][PubMed]
     [Google Scholar]
  18. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018; 68:461466 [View Article][PubMed]
     [Google Scholar]
  19. Thompson CC, Chimetto L, Edwards RA, Swings J, Stackebrandt E et al. Microbial genomic taxonomy. BMC Genomics 2013; 14:913 [View Article][PubMed]
     [Google Scholar]
  20. Logan NA, Vos PD. The genus Bacillus . In Vos PD, Garrity GM, Jones D, Krieg ND, Ludwig W et al. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed, vol. 3, The Firmicutes New York: Springer; 2009 p 62.
     [Google Scholar]
  21. Zheng B-X, Ibrahim M, Zhang D-P, Bi Q-F, Li H-Z et al. Identification and characterization of inorganic-phosphate-solubilizing bacteria from agricultural fields with a rapid isolation method. AMB Express 2018; 8:47 [View Article][PubMed]
     [Google Scholar]
  22. Brenner DJ, Steigerwalt AG, Gorman GW, Wilkinson HW, Bibb WF et al. Ten new species of Legionella . Int J Syst Bacteriol 1985; 35:50–59 [View Article]
     [Google Scholar]
  23. Aslanzadeh J. Biochemical profile-based microbial identification systems. In Tang YW, Stratton CW. (editors) (editors) Advanced Techniques in Diagnostic Microbiology New York: Springer; 2013 pp 87–121
     [Google Scholar]
  24. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. USFCC News Lett 1990; 20:1–6
     [Google Scholar]
  25. Yang G, Benson RF, Ratcliff RM, Brown EW, Steigerwalt AG et al. Legionella nagasakiensis sp. nov., isolated from water samples and from a patient with pneumonia. Int J Syst Evol Microbiol 2012; 62:284–288 [View Article][PubMed]
     [Google Scholar]
  26. Kuroki H, Miyamoto H, Fukuda K, Iihara H, Kawamura Y et al. Legionella impletisoli sp. nov. and Legionella yabuuchiae sp. nov., isolated from soils contaminated with industrial wastes in Japan. Syst Appl Microbiol 2007; 30:273–279 [View Article][PubMed]
     [Google Scholar]
  27. Dennis PJ, Brenner DJ, Thacker WL, Wait R, Vesey G et al. Five new Legionella species isolated from water. Int J Syst Bacteriol 1993; 43:329–337 [View Article][PubMed]
     [Google Scholar]
  28. Benson RF, Thacker WL, Lanser JA, Sangster N, Mayberry WR et al. Legionella adelaidensis, a new species isolated from cooling tower water. J Clin Microbiol 1991; 29:1004–1006 [View Article][PubMed]
     [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.004592
Loading
Legionella septentrionalis sp. nov., isolated from aquatic environments in the northern PR China
Int J Syst Evol Microbiol 71, 004592 (2020); https://doi.org/10.1099/ijsem.0.004592
/content/journal/ijsem/10.1099/ijsem.0.004592
/content/journal/ijsem/10.1099/ijsem.0.004592
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error