@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004592, author = "Li, Liang-Hui and Zhang, Lei and Wu, Hai-Yan and Qu, Ping-Hua and Chen, Jia-Chang and Zhan, Xiao-Yong and Zhu, Qing-Yi and Chen, Cha and Hu, Chao-Hui", title = "Legionella septentrionalis sp. nov., isolated from aquatic environments in the northern PR China", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "71", number = "1", pages = "", doi = "https://doi.org/10.1099/ijsem.0.004592", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004592", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Legionella septentrionalis sp. nov.", keywords = "aquatic environments", keywords = "polyphasic taxonomy", eid = "004592", abstract = "Four strains (km711T, km714, km542 and km524), representing a novel Legionella species, were isolated from aquatic environments in northern PR China. Cells were Gram-stain-negative, rod-shaped, microaerobic, motile and growth depended on l-cysteine. They grew at 25‒42 °C (optimum, 35‒37 °C) and could tolerate up to 1.5 % (w/v) NaCl (optimum, 0.5 %). The major fatty acids (>5 %) of the type strain km711T were C17 : 0 anteiso, C15 : 0 anteiso, iso-C16 : 0 and C16 : 1  ω7c and/or iso-C15 : 0 2OH. The pairwise comparison values were <96.1 % for 16S rRNA gene sequences, 23.3‒28.7 % interspecies variation for mip gene sequences, <93.6 % average nucleotide identity and <72.8 % average amino acid identity between these four strains and related type strains within the genus Legionella . The phylogenetic tree based on the four concatenated genes (16S rRNA, mip, rpoB and rnpB) and protein-concatamer tree based on concatenation of 21 protein markers both revealed that these four strains formed a separate phylogenetic branch cluster within the genus Legionella . The results of phenotypic and genotypic features suggest that these four strains represent a novel species of the genus Legionella , for which the name Legionella septentrionalis sp. nov. is proposed (type strain km711T=KCTC 15655T=NBRC 113219T).", }