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Volume 70, Issue 12

sp. nov., isolated from a bacterial consortia of contaminated soil enriched for the remediation of e-waste Free

Abstract

A novel e-waste-degrading strain, PE08, was isolated from contaminated soil collected from a paper mill yard in Lalkuan, Uttarakhand, India. Strain PE08 was Gram-stain-negative, rod-shaped, aerobic, oxidase-positive and catalase-positive. Optimum growth was observed at 30 °C (range, 5–40 °C), with 1–2 % NaCl (range, 0–3 %) and at pH 7 (range 6–11). The phylogeny based on 16S rRNA gene sequences delineated strain PE08 to the genus and showed highest sequence similarity to KF707 (98.70 %), followed by DSM 50071 (98.62 %) and DSM 21078 (97.93 %). The genome of strain PE08 was sequenced and had one scaffold of 6056953 bp, 99.84 % completeness and 182× coverage were obtained. The G+C content in the genome was 64.24 mol%. The DNA–DNA hybridization and average nucleotide identity values between strain PE08 and its closely related type strain, DSM 21078 were below 34.8 % and 87.96 %, respectively. The phylogenetic analysis based on whole-genome sequence and concatenated GyrB and RpoB proteins revealed that strain PE08 forms a district clade in the family . The predominant fatty acids were summed feature 8 (Cω7 and/or C ω6), summed feature 3 (Cω7 and/or Cω6), C and C. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The phenotypic, chemotaxonomic and genetic analysis, including overall genome relatedness index values, indicated that strain PE08 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PE08 (=MCC 3792=KCTC 72454=CCUG 73691).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bioremediation , degradation , e-waste , Pseudomonas and soil bacteria
Funding
This study was supported by the:
  • University Grants Commission (IN) (Award 857/CSIR-UGC NET JUNE 2017)
  • Department of Science and Technology, Ministry of Science and Technology, http://dx.doi.org/10.13039/501100001409 (Award SRG/2019/001818)
  • Department of Biotechnology, Ministry of Science and Technology, http://dx.doi.org/10.13039/501100001407 (Award BT/COORD.II/01/03/2016)
  • Science and Engineering Research Board, http://dx.doi.org/10.13039/501100001843 (Award SERB/EEQ/2016/000752)
© 2020 The Authors
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2020-11-11
2024-09-18
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Pseudomonas lalkuanensis sp. nov., isolated from a bacterial consortia of contaminated soil enriched for the remediation of e-waste
Int J Syst Evol Microbiol 70, 6468 (2020); https://doi.org/10.1099/ijsem.0.004559
/content/journal/ijsem/10.1099/ijsem.0.004559
/content/journal/ijsem/10.1099/ijsem.0.004559
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