%0 Journal Article %A Thorat, Vipool %A Kirdat, Kiran %A Tiwarekar, Bhavesh %A DaCosta, Elaine %A Debbarma, Prasenjit %A Shouche, Yogesh %A Sathe, Shivaji %A Goel, Reeta %A Lodha, Tushar %A Yadav, Amit %T Pseudomonas lalkuanensis sp. nov., isolated from a bacterial consortia of contaminated soil enriched for the remediation of e-waste %D 2020 %J International Journal of Systematic and Evolutionary Microbiology, %V 70 %N 12 %P 6468-6475 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.004559 %K degradation %K soil bacteria %K Pseudomonas %K bioremediation %K e-waste %I Microbiology Society, %X A novel e-waste-degrading strain, PE08T, was isolated from contaminated soil collected from a paper mill yard in Lalkuan, Uttarakhand, India. Strain PE08T was Gram-stain-negative, rod-shaped, aerobic, oxidase-positive and catalase-positive. Optimum growth was observed at 30 °C (range, 5–40 °C), with 1–2 % NaCl (range, 0–3 %) and at pH 7 (range 6–11). The phylogeny based on 16S rRNA gene sequences delineated strain PE08T to the genus Pseudomonas and showed highest sequence similarity to Pseudomonas furukawaii KF707T (98.70 %), followed by Pseudomonas aeruginosa DSM 50071T (98.62 %) and Pseudomonas resinovorans DSM 21078T (97.93 %). The genome of strain PE08T was sequenced and had one scaffold of 6056953 bp, 99.84 % completeness and 182× coverage were obtained. The G+C content in the genome was 64.24 mol%. The DNA–DNA hybridization and average nucleotide identity values between strain PE08T and its closely related type strain, P. resinovorans DSM 21078T were below 34.8 % and 87.96 %, respectively. The phylogenetic analysis based on whole-genome sequence and concatenated GyrB and RpoB proteins revealed that strain PE08T forms a district clade in the family Pseudomonadaceae . The predominant fatty acids were summed feature 8 (C18 :  1ω7c and/or C18 :1 ω6c), summed feature 3 (C16 :  1ω7c and/or C16 :  1ω6c), C16 : 0 and C12 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The phenotypic, chemotaxonomic and genetic analysis, including overall genome relatedness index values, indicated that strain PE08T represents a novel species of the genus Pseudomonas , for which the name Pseudomonas lalkuanensis sp. nov. is proposed. The type strain is PE08T (=MCC 3792=KCTC 72454=CCUG 73691). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004559