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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 12

sp. nov., a galactomannan-degrading haloarchaeon isolated from commercial salt Free

Abstract

A mannan-degrading halophilic archaeal strain, MD130-1, was isolated from a commercial salt sample. Cells were motile, rod-shaped, and stained Gram-negative. Colonies were pink pigmented. Strain MD130-1 was able to grow at 1.5–4.6 M NaCl (optimum, 3.6 M) at pH 6.0–8.0 (optimum, pH 7.0) and at 25–50 °C (optimum, 40 °C). The DNA G+C content was 62.1 mol% (genome). The orthologous 16S rRNA gene sequence showed the highest similarity (99.4 %) to those of JCM 7785 and JCM 8911. The values of genome relatedness between strain MD130-1 and species were 84.33–85.96 % in ANIb and 30.4–32.9 % using GGDC formula 2. The polar lipids of strain MD130-1 were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and triglycosyl diether-2. Based on the results of phenotypic and phylogenetic analyses, the strain represents a new species of the genus , for which the name sp. nov. is proposed. The type strain is MD130-1 (=JCM 33835=KCTC 4287) isolated from commercial salt made in Ishikawa prefecture, Japan.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): galactomannan degrading , Haloarcula and Haloarcula mannanilytica
Funding
This study was supported by the:
  • The Salt Science Research Foundation (Award No. 1445)
    • Principle Award Recipient: Hiroaki Minegishi
© 2020 The Authors
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2020-10-23
2023-09-28
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Haloarcula mannanilytica sp. nov., a galactomannan-degrading haloarchaeon isolated from commercial salt
Int J Syst Evol Microbiol 70, 6331 (2020); https://doi.org/10.1099/ijsem.0.004535
/content/journal/ijsem/10.1099/ijsem.0.004535
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