%0 Journal Article %A Enomoto, Shigeaki %A Shimane, Yasuhiro %A Ihara, Kunio %A Kamekura, Masahiro %A Itoh, Takashi %A Ohkuma, Moriya %A Takahashi-Ando, Naoko %A Fukushima, Yasumasa %A Yoshida, Yasuhiko %A Usami, Ron %A Takai, Ken %A Minegishi, Hiroaki %T Haloarcula mannanilytica sp. nov., a galactomannan-degrading haloarchaeon isolated from commercial salt %D 2020 %J International Journal of Systematic and Evolutionary Microbiology, %V 70 %N 12 %P 6331-6337 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.004535 %K Haloarcula %K galactomannan degrading %K Haloarcula mannanilytica %I Microbiology Society, %X A mannan-degrading halophilic archaeal strain, MD130-1T, was isolated from a commercial salt sample. Cells were motile, rod-shaped, and stained Gram-negative. Colonies were pink pigmented. Strain MD130-1T was able to grow at 1.5–4.6 M NaCl (optimum, 3.6 M) at pH 6.0–8.0 (optimum, pH 7.0) and at 25–50 °C (optimum, 40 °C). The DNA G+C content was 62.1 mol% (genome). The orthologous 16S rRNA gene sequence showed the highest similarity (99.4 %) to those of Haloarcula japonica JCM 7785T and Haloarcula hispanica JCM 8911T. The values of genome relatedness between strain MD130-1T and Haloarcula species were 84.33–85.96 % in ANIb and 30.4–32.9 % using GGDC formula 2. The polar lipids of strain MD130-1T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and triglycosyl diether-2. Based on the results of phenotypic and phylogenetic analyses, the strain represents a new species of the genus Haloarcula , for which the name Haloarcula mannanilytica sp. nov. is proposed. The type strain is MD130-1T (=JCM 33835T=KCTC 4287T) isolated from commercial salt made in Ishikawa prefecture, Japan. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004535