1887

Abstract

We carried out a polyphasic taxonomic study on a new halophilic strain designated 3(2), isolated from Meighan wetland, Iran. Cells of the novel strain were Gram-stain-negative, non-hemolytic, catalase- and oxidase-positive, rod-shaped, non-endospore-forming and motile. Cell growth occurred at 3–15 % NaCl (w/v; optimum, 5 %), pH 7.0–9.0 (optimum, pH 7.5–8.0) and 15–35 °C (optimum, 30 °C). 16S rRNA gene sequence comparisons confirmed the affiliation of strain 3(2) to the class and the genus with highest similarity to YCSA28 (98.4 %) and Al12 (97.9 %). Experimental and DNA–DNA hybridization values were 42.7 and 35.1% with IBRC-M 10931 and 48 and 35.2% with IBRC-M 10566, respectively, and indicated that they are different members of the same genus. The genome of the type strain was characterized by a size of 3.83 Mbp with 63 scaffolds and a G+C content of 64.8 mol%. Moreover, the average nucleotide identity values against Al12 and YCSA28 were 88.8 and 88.5 %, respectively. The predominant respiratory quinone was Q-9 (92 %) with Q-8 (8 %) as a minor component. Major fatty acids were C cyclo, C ω8, C ω7 and/or iso-C2-OH, C 3-OH and C ω7. The polar lipid profile of the strain contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphoaminoglycolipid and four unidentified phospholipids. According to our results, strain 3(2) could be classified as a novel species in the genus for which the name sp. nov. is proposed. The type strain is 3(2) (=IBRC M 10929=LMG 29450=CIP 111708).

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2020-10-14
2021-02-26
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