1887

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Volume 70, Issue 11

sp. nov., isolated from freshwater Free

Abstract

A Gram-stain-negative, rod-shaped, obligately aerobic, motile by a single polar flagellum, chemoheterotrophic bacterium, designated strain IMCC25680, was isolated from surface water in Chungju Lake, Republic of Korea. 16S rRNA gene sequence analysis revealed that strain IMCC25680 was most closely related to HW7 with 95.5% sequence similarity and formed a robust clade with HW7. Whole genome sequencing showed that strain IMCC25680 had a genome 3.6 Mbp long with 60.7 mol% DNA G+C content. Average nucleotide identity and digital DNA–DNA hybridization values between strain IMCC25680 and HW7 were 72.4% and 18.5%, respectively, indicating that the novel strain represents a novel species of the genus . The major cellular fatty acids of strain IMCC25680 were iso-C and summed feature 3 (comprising C 6 and/or C 7). The respiratory quinone detected in the strain was ubiquinone-8. The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified polar lipids. On the basis of the phylogenetic and phenotypic characterization, strain IMCC25680 was considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is IMCC25680 (=KACC 19487 =NBRC 113132).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): freshwater , genome , Leeia , Leeia aquaticae , polyphasic and taxonomy
Funding
This study was supported by the:
  • Ministry of Education, Science and Technology (Award 2019R1A2B5B02070538)
    • Principle Award Recipient: Jang-Cheon Cho
© 2020 The Authors
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2020-09-28
2024-05-13
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Leeia aquatica sp. nov., isolated from freshwater
Int J Syst Evol Microbiol 70, 5848 (2020); https://doi.org/10.1099/ijsem.0.004487
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