@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004487, author = "Song, Jaeho and Joung, Yochan and Li, Shan-Hui and Hwang, Juchan and Cho, Jang-Cheon", title = "Leeia aquatica sp. nov., isolated from freshwater", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "11", pages = "5848-5853", doi = "https://doi.org/10.1099/ijsem.0.004487", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004487", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "genome", keywords = "Leeia aquaticae", keywords = "freshwater", keywords = "polyphasic", keywords = "taxonomy", keywords = "Leeia", abstract = "A Gram-stain-negative, rod-shaped, obligately aerobic, motile by a single polar flagellum, chemoheterotrophic bacterium, designated strain IMCC25680T, was isolated from surface water in Chungju Lake, Republic of Korea. 16S rRNA gene sequence analysis revealed that strain IMCC25680T was most closely related to Leeia oryzae HW7T with 95.5% sequence similarity and formed a robust clade with L. oryzae HW7T. Whole genome sequencing showed that strain IMCC25680T had a genome 3.6 Mbp long with 60.7 mol% DNA G+C content. Average nucleotide identity and digital DNA–DNA hybridization values between strain IMCC25680T and L. oryzae HW7T were 72.4% and 18.5%, respectively, indicating that the novel strain represents a novel species of the genus Leeia . The major cellular fatty acids of strain IMCC25680T were iso-C16:0 and summed feature 3 (comprising C16:1 ω6c and/or C16:1 ω7c). The respiratory quinone detected in the strain was ubiquinone-8. The major polar lipids were found to be phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified polar lipids. On the basis of the phylogenetic and phenotypic characterization, strain IMCC25680T was considered to represent a novel species within the genus Leeia , for which the name Leeia aquatica sp. nov. is proposed. The type strain is IMCC25680T (=KACC 19487T =NBRC 113132T).", }