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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 10

sp. nov. and sp. nov., two novel species of the family isolated from the South China Sea Free

Abstract

Strains J15B81-2 and J15B91 were isolated from a sediment sample collected from the South China Sea. Cells of both strains were observed to be rod-shaped, non-gliding, Gram-stain-negative, yellow-pigmented, facultatively anaerobic, catalase-positive, oxidase-negative and showing optimum growth at 30 °C. Strains J15B81-2 and J15B91 could tolerate up to 9 and 10  % (w/v) NaCl concentration and grow at pH 6.5–9.5 and 6.0–9.0, respectively. The strains shared 97.4 % 16S rRNA gene sequence similarity to each other but were identified as two distinct species based on 81.1–85.8 % ANIb and 31.5 % dDDH values calculated using whole genome sequences. Strains J15B81-2 and J15B91 shared highest 16S rRNA gene sequence similarity to CGMCC 1.12522 (98.4 %) and CGMCC 1.12641 (98.0 %), respectively. Among species with validly published names, CGMCC 1.12641 shared close genetic relatedness with strains J15B81-2 [85.1–85.3% average nucleotide identity based on + (ANIb) and 30.6 % digital DNA–DNA hybridization (dDDH)] and J15B91 (76.6–79.1 % ANIb and 21.5 % dDDH). The major fatty acid of strains J15B81-2 and J15B91 were identified as iso-C and iso-C, respectively, and the major polar lipids of the two strains consisted of phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and one unidentified lipid. The strains contained MK-6 as their predominant menaquinone. The genomic G+C contents of strains J15B81-2 and J15B91 were determined to be 41.7 and 41.8 mol %, respectively. Both strains are considered to represent two novel species of the genus and the names sp. nov. and sp. nov. are proposed for strains J15B81-2 (=KCTC 72867=MCCC 1H00410) and J15B91 (=KCTC 72869=MCCC 1H00411), respectively.

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Keyword(s): Bacteroidetes , Flavobacteriaceae , polyphasic taxonomy , Salinimicrobium nanhaiense sp. nov. and Salinimicrobium oceani sp. nov.
Funding
This study was supported by the:
  • National Natural Science Foundation of China, http://dx.doi.org/10.13039/501100001809 (Award 41976202)
  • National Natural Science Foundation of China, http://dx.doi.org/10.13039/501100001809 (Award 41576064)
  • State Key Laboratory of Microbial Technology Open Projects Fund (Award M2020-03)
  • Open Fund of the Key Laboratory of Marine Geology and Environment, Chinese Academy of Sciences (Award MGE2020KG14)
  • Fundamental Research Funds of Shandong University
© 2020 The Authors
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2020-08-21
2024-05-14
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Salinimicrobium nanhaiense sp. nov. and Salinimicrobium oceani sp. nov., two novel species of the family Flavobacteriaceae isolated from the South China Sea
Int J Syst Evol Microbiol 70, 5263 (2020); https://doi.org/10.1099/ijsem.0.004405
/content/journal/ijsem/10.1099/ijsem.0.004405
/content/journal/ijsem/10.1099/ijsem.0.004405
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