1887

Abstract

Strains J15B81-2 and J15B91 were isolated from a sediment sample collected from the South China Sea. Cells of both strains were observed to be rod-shaped, non-gliding, Gram-stain-negative, yellow-pigmented, facultatively anaerobic, catalase-positive, oxidase-negative and showing optimum growth at 30 °C. Strains J15B81-2 and J15B91 could tolerate up to 9 and 10  % (w/v) NaCl concentration and grow at pH 6.5–9.5 and 6.0–9.0, respectively. The strains shared 97.4 % 16S rRNA gene sequence similarity to each other but were identified as two distinct species based on 81.1–85.8 % ANIb and 31.5 % dDDH values calculated using whole genome sequences. Strains J15B81-2 and J15B91 shared highest 16S rRNA gene sequence similarity to CGMCC 1.12522 (98.4 %) and CGMCC 1.12641 (98.0 %), respectively. Among species with validly published names, CGMCC 1.12641 shared close genetic relatedness with strains J15B81-2 [85.1–85.3% average nucleotide identity based on + (ANIb) and 30.6 % digital DNA–DNA hybridization (dDDH)] and J15B91 (76.6–79.1 % ANIb and 21.5 % dDDH). The major fatty acid of strains J15B81-2 and J15B91 were identified as iso-C and iso-C, respectively, and the major polar lipids of the two strains consisted of phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and one unidentified lipid. The strains contained MK-6 as their predominant menaquinone. The genomic G+C contents of strains J15B81-2 and J15B91 were determined to be 41.7 and 41.8 mol %, respectively. Both strains are considered to represent two novel species of the genus and the names sp. nov. and sp. nov. are proposed for strains J15B81-2 (=KCTC 72867=MCCC 1H00410) and J15B91 (=KCTC 72869=MCCC 1H00411), respectively.

Funding
This study was supported by the:
  • , National Natural Science Foundation of China, http://dx.doi.org/10.13039/501100001809, (Award 41976202)
  • , National Natural Science Foundation of China, http://dx.doi.org/10.13039/501100001809, (Award 41576064)
  • , State Key Laboratory of Microbial Technology Open Projects Fund , (Award M2020-03)
  • , Open Fund of the Key Laboratory of Marine Geology and Environment, Chinese Academy of Sciences , (Award MGE2020KG14)
  • , Fundamental Research Funds of Shandong University
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2020-08-21
2021-02-26
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