@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004405, author = "Cao, Wen-Rui and Zhang, Lan-Zeng and Hu, Yi-Hao and Jiang, Ming-Yu and Li, Ying-Jie", title = "Salinimicrobium nanhaiense sp. nov. and Salinimicrobium oceani sp. nov., two novel species of the family Flavobacteriaceae isolated from the South China Sea", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "10", pages = "5263-5270", doi = "https://doi.org/10.1099/ijsem.0.004405", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004405", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Salinimicrobium oceani sp. nov.", keywords = "polyphasic taxonomy", keywords = "Flavobacteriaceae", keywords = "Bacteroidetes", keywords = "Salinimicrobium nanhaiense sp. nov.", abstract = "Strains J15B81-2T and J15B91T were isolated from a sediment sample collected from the South China Sea. Cells of both strains were observed to be rod-shaped, non-gliding, Gram-stain-negative, yellow-pigmented, facultatively anaerobic, catalase-positive, oxidase-negative and showing optimum growth at 30 °C. Strains J15B81-2T and J15B91T could tolerate up to 9 and 10  % (w/v) NaCl concentration and grow at pH 6.5–9.5 and 6.0–9.0, respectively. The strains shared 97.4 % 16S rRNA gene sequence similarity to each other but were identified as two distinct species based on 81.1–85.8 % ANIb and 31.5 % dDDH values calculated using whole genome sequences. Strains J15B81-2T and J15B91T shared highest 16S rRNA gene sequence similarity to Salinimicrobium xinjiangense CGMCC 1.12522T (98.4 %) and Salinimicrobium sediminis CGMCC 1.12641T (98.0 %), respectively. Among species with validly published names, S. sediminis CGMCC 1.12641T shared close genetic relatedness with strains J15B81-2T [85.1–85.3% average nucleotide identity based on blastBlast+ (ANIb) and 30.6 % digital DNA–DNA hybridization (dDDH)] and J15B91T (76.6–79.1 % ANIb and 21.5 % dDDH). The major fatty acid of strains J15B81-2T and J15B91T were identified as iso-C15 : 0 and iso-C16 : 0, respectively, and the major polar lipids of the two strains consisted of phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and one unidentified lipid. The strains contained MK-6 as their predominant menaquinone. The genomic G+C contents of strains J15B81-2T and J15B91T were determined to be 41.7 and 41.8 mol %, respectively. Both strains are considered to represent two novel species of the genus Salinimicrobium and the names Salinimicrobium nanhaiense sp. nov. and Salinimicrobium oceani sp. nov. are proposed for strains J15B81-2T (=KCTC 72867T=MCCC 1H00410T) and J15B91T (=KCTC 72869T=MCCC 1H00411T), respectively.", }