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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 9

sp. nov., a novel actinobacterium isolated from faeces Free

Abstract

A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain, designated YIM 102482-1, was isolated from the faeces of . Strain YIM 102482-1 grew optimally at 30–37 °C, at pH 8.0 and in the presence of 1.0–3.0% (w/v) NaCl. Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1 was a member of the genus and most closely related to NBRC 15706 (97.6 %), NBRC 103089 (97.6 %), KCTC 13959 (96.4 %) and DSM 13485 (96.0 %), respectively. Furthermore, phylogenetic trees based on 16S rRNA gene sequences and genomic sequences demonstrated that strain YIM 102482-1 formed a distinct branch with all type strains of the genus . The major whole-cell sugars and cellular fatty acids (>10.0 %) were ribose and rhamnose, and anteiso-C, iso-C and C, respectively. The predominant menaquinone was MK-9, and 2,4-diaminobutyric acid and ornithine were the diagnostic diamino acids in the cell-wall peptidoglycan. The dominant polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and unidentified glycolipid. The DNA G+C content of YIM 102482-1 was 63.0 mol%. Based on analysis results of physiological, biochemical and chemotaxonomic data, strain YIM 102482-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 102482-1(=DSM 102156=CCTCC AB 2016023).

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Keyword(s): faeces , Gulosibacter macacae sp. nov. and Macaca mulatta
Funding
This study was supported by the:
  • National Natural Science Foundation of China, http://dx.doi.org/10.13039/501100001809 (Award 81573327)
  • National Natural Science Foundation of China, http://dx.doi.org/10.13039/501100001809 (Award 31460005)
  • National Natural Science Foundation of China (Award 31270001)
© 2020 The Authors
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2020-08-18
2025-05-16
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Gulosibacter macacae sp. nov., a novel actinobacterium isolated from Macaca mulatta faeces
Int J Syst Evol Microbiol 70, 5115 (2020); https://doi.org/10.1099/ijsem.0.004389
/content/journal/ijsem/10.1099/ijsem.0.004389
/content/journal/ijsem/10.1099/ijsem.0.004389
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