Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 6

sp. nov., isolated from the human mouth Free

Abstract

Three strains representing the previously uncultured human oral taxon HMT-286 were recently isolated from the subgingival plaque of a patient with chronic periodontitis. The phenotypic and genetic features of strain SP18_26 were compared to those of the type species of , . A genome size of 2.97 Mbp (G+C content 56.5 mol%) was previously reported for SP18_26, compared to a size of 3.28 Mbp (47.1 mol%) in ATCC 43037. 16S rRNA gene sequence comparisons also revealed 94.3 % sequence identity with ATCC 43037. Growth was stimulated by supplementation of media with -acetyl muramic acid, as seen with , but the cells displayed a distinctive snake-like morphology. Fatty acid methyl ester analysis revealed a profile differing from , chiefly in the amount of 3-OH-16 : 0 (four-fold lower in SP18_26). Overall, metabolic enzyme activity also differed from , with enzyme activity for indole present, but the complement of glycoside hydrolase enzyme activity was smaller than , for example, lacking sialidase and -acetyl-β-glucosaminidase – evidence backed up by analysis of its gene content. On the basis of these results, a new species is proposed for which the type strain is SP18_26 (=DSM 102894=JCM 31303).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): HMT-286 , oral microbiology , periodontitis , Porphyromonadaceae , subgingival plaque and Tannerella
© 2020 The Authors
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004229
2020-06-10
2025-01-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/70/6/3749.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.004229&mimeType=html&fmt=ahah

References

  1. Tanner ACR, Listgarten MA, Ebersole JL, Strzempko MN. Bacteroides sp. from the human oral cavity. Cell 1986213–221
     [Google Scholar]
  2. Paster BJ, Boches SK, Galvin JL, Ericson RE, Lau CN et al. Bacterial diversity in human subgingival plaque. J Bacteriol 2001; 183:3770–3783 [View Article]
     [Google Scholar]
  3. Leys EJ, Lyons SR, Moeschberger ML, Rumpf RW, Griffen AL. Association of Bacteroides forsythus and a novel Bacteroides phylotype with periodontitis. J Clin Microbiol 2002; 40:821–825 [View Article]
     [Google Scholar]
  4. Vartoukian SR, Moazzez RV, Paster BJ, Dewhirst FE, Wade WG. First Cultivation of Health-Associated Tannerella sp. HOT-286 (BU063). J Dent Res 2016; 95:1308–1313 [View Article]
     [Google Scholar]
  5. de Lillo A, Booth V, Kyriacou L, Weightman AJ, Wade WG. Culture-Independent identification of periodontitis-associated Porphyromonas and Tannerella populations by targeted molecular analysis. J Clin Microbiol 2004; 42:5523–5527 [View Article]
     [Google Scholar]
  6. Kumar PS, Griffen AL, Barton JA, Paster BJ, Moeschberger ML et al. New bacterial species associated with chronic periodontitis. J Dent Res 2003; 82:338–344 [View Article]
     [Google Scholar]
  7. Frey AM, Ansbro K, Kamble NS, Pham TK, Stafford GP. Characterisation and pure culture of putative health-associated oral bacterium BU063 (Tannerella sp. HOT-286) reveals presence of a potentially novel glycosylated S-layer. FEMS Microbiol Lett 2018; 365:1–8 [View Article]
     [Google Scholar]
  8. Beall CJ, Campbell AG, Griffen AL, Podar M, Leys EJ. Genomics of the uncultivated, periodontitis-associated bacterium Tannerella sp. BU045 (Oral Taxon 808). mSystems 2018; 3:1–11
     [Google Scholar]
  9. Lefort V, Desper R, Gascuel O. FastME 2.0: a comprehensive, accurate, and fast Distance-Based phylogeny inference program: table 1. Mol Biol Evol 2015; 32:2798–2800 [View Article]
     [Google Scholar]
  10. Meier-Kolthoff JP, Göker M. TYGS is an automated high-throughput platform for state-of-the-art genome-based taxonomy. Nat Commun 2019; 10:2182 [View Article]
     [Google Scholar]
  11. Beall CJ, Campbell AG, Dayeh DM, Griffen AL, Podar M et al. Single cell genomics of uncultured, health-associated Tannerella BU063 (oral taxon 286) and comparison to the closely related pathogen Tannerella forsythia. PLoS One 2014; 9:e89398 [View Article]
     [Google Scholar]
  12. Friedrich V, Pabinger S, Chen T, Messner P, Dewhirst FE et al. Draft Genome Sequence of Tannerella forsythia Type Strain ATCC 43037. Genome Announc 2015; 3: [View Article]
     [Google Scholar]
  13. Rodriguez-R LM, Gunturu S, Harvey WT, Rosselló-Mora R, Tiedje JM et al. The microbial genomes atlas (MiGA) Webserver: taxonomic and gene diversity analysis of archaea and bacteria at the whole genome level. Nucleic Acids Res 2018; 46:W282–W288 [View Article]
     [Google Scholar]
  14. Gascuel O. BIONJ: an improved version of the NJ algorithm based on a simple model of sequence data. Mol Biol Evol 1997; 14:685–695 [View Article]
     [Google Scholar]
  15. Segata N, Börnigen D, Morgan XC, Huttenhower C. PhyloPhlAn is a new method for improved phylogenetic and taxonomic placement of microbes. Nat Commun 2013; 4:2304 [View Article]
     [Google Scholar]
  16. Wattam AR, Abraham D, Dalay O, Disz TL, Driscoll T et al. PATRIC, the bacterial bioinformatics database and analysis resource. Nucleic Acids Res 2014; 42:D581–D591 [View Article]
     [Google Scholar]
  17. Zwickl NF, Stralis-Pavese N, Schäffer C, Dohm JC, Himmelbauer H. Comparative genome characterization of the periodontal pathogen Tannerella forsythia. BMC Genomics 2020; 21:150 [View Article]
     [Google Scholar]
  18. Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B. The carbohydrate-active enzymes database (CAZy) in 2013. Nucleic Acids Res 2014; 42:D490–D495 [View Article]
     [Google Scholar]
  19. Sakamoto M, Suzuki M, Umeda M, Ishikawa I, Benno Y. Reclassification of Bacteroides forsythus (Tanner, et al. 1986) as Tannerella forsythensis corrig., gen. nov., comb. nov. Int J Syst Evol Microbiol 2002; 52:841–849
     [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.004229
Loading
Tannerella serpentiformis sp. nov., isolated from the human mouth
Int J Syst Evol Microbiol 70, 3749 (2020); https://doi.org/10.1099/ijsem.0.004229
/content/journal/ijsem/10.1099/ijsem.0.004229
/content/journal/ijsem/10.1099/ijsem.0.004229
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error