1887

Abstract

Three strains representing the previously uncultured human oral taxon HMT-286 were recently isolated from the subgingival plaque of a patient with chronic periodontitis. The phenotypic and genetic features of strain SP18_26 were compared to those of the type species of , . A genome size of 2.97 Mbp (G+C content 56.5 mol%) was previously reported for SP18_26, compared to a size of 3.28 Mbp (47.1 mol%) in ATCC 43037. 16S rRNA gene sequence comparisons also revealed 94.3 % sequence identity with ATCC 43037. Growth was stimulated by supplementation of media with -acetyl muramic acid, as seen with , but the cells displayed a distinctive snake-like morphology. Fatty acid methyl ester analysis revealed a profile differing from , chiefly in the amount of 3-OH-16 : 0 (four-fold lower in SP18_26). Overall, metabolic enzyme activity also differed from , with enzyme activity for indole present, but the complement of glycoside hydrolase enzyme activity was smaller than , for example, lacking sialidase and -acetyl-β-glucosaminidase – evidence backed up by analysis of its gene content. On the basis of these results, a new species is proposed for which the type strain is SP18_26 (=DSM 102894=JCM 31303).

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/content/journal/ijsem/10.1099/ijsem.0.004229
2020-06-10
2020-12-01
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