@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004229, author = "Ansbro, Katherine and Wade, William G. and Stafford, Graham P.", title = "Tannerella serpentiformis sp. nov., isolated from the human mouth", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "6", pages = "3749-3754", doi = "https://doi.org/10.1099/ijsem.0.004229", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004229", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "subgingival plaque", keywords = "Tannerella", keywords = "periodontitis", keywords = "oral microbiology", keywords = "HMT-286", keywords = "Porphyromonadaceae", abstract = "Three strains representing the previously uncultured human oral Tannerella taxon HMT-286 were recently isolated from the subgingival plaque of a patient with chronic periodontitis. The phenotypic and genetic features of strain SP18_26T were compared to those of the type species of Tannerella , Tannerella forsythia . A genome size of 2.97 Mbp (G+C content 56.5 mol%) was previously reported for SP18_26T, compared to a size of 3.28 Mbp (47.1 mol%) in T. forsythia ATCC 43037T. 16S rRNA gene sequence comparisons also revealed 94.3 % sequence identity with T. forsythia ATCC 43037T. Growth was stimulated by supplementation of media with N-acetyl muramic acid, as seen with T. forsythia , but the cells displayed a distinctive snake-like morphology. Fatty acid methyl ester analysis revealed a profile differing from T. forsythia , chiefly in the amount of 3-OH-16 : 0 (four-fold lower in SP18_26T). Overall, metabolic enzyme activity also differed from T. forsythia , with enzyme activity for indole present, but the complement of glycoside hydrolase enzyme activity was smaller than T. forsythia , for example, lacking sialidase and N-acetyl-β-glucosaminidase – evidence backed up by analysis of its gene content. On the basis of these results, a new species Tannerella serpentiformis is proposed for which the type strain is SP18_26T (=DSM 102894T=JCM 31303T).", }