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Abstract

A yellow-pigmented, Gram-stain-negative, aerobic, non-motile rod shaped, mesophilic bacterium, designated strain N7XX-4, was isolated from cattail root grown on the mine tailings of Phoenix mountain, Tongling city, Anhui Province (PR China). Analysis of the 16S rRNA gene sequence revealed that the strain represented a novel member of the family . The nearest phylogenetic neighbour was MSL-13 (97.8 % 16S rRNA gene sequence similarity). The most abundant fatty acid in whole cells of N7XX-4 was anteiso-C15 : 0 (29.9 %). The predominant menaquinones were MK-12(H), MK-13(H) and MK-11(H). The peptidoglycan type of the isolate was B1δ with -Lys as the diagnostic cell-wall diamino acid. On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4 (=CGMCC 1.16548=DSM 106791=JCM 32630) is designated as the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

Funding
This study was supported by the:
  • National Basic Research Program of China (973 Program) (Award 2015CB150503)
    • Principle Award Recipient: Shixue Yin
  • National Natural Science Foundation of China (Award 41071177)
    • Principle Award Recipient: Shixue Yin
  • Environmental Protection of Yangzhou City (Award YHK1414)
    • Principle Award Recipient: Lijuan Mei
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2020-01-28
2024-12-06
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References

  1. Jang Y-H, Kim S-J, Tamura T, Hamada M, Weon H-Y et al. Lysinimonas soli gen. nov., sp. nov., isolated from soil, and reclassification of Leifsonia kribbensis Dastager et al. 2009 as Lysinimonas kribbensis sp. nov., comb. nov. Int J Syst Evol Microbiol 2013; 63:1403–1410 [View Article]
    [Google Scholar]
  2. Stevenson BS, Eichorst SA, Wertz JT, Schmidt TM, Breznak JA. New strategies for cultivation and detection of previously uncultured microbes. Appl Environ Microbiol 2004; 70:4748–4755 [View Article]
    [Google Scholar]
  3. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp 607–654
    [Google Scholar]
  4. Tarrand JJ, Gröschel DH, Rapid GDH. Rapid, modified oxidase test for oxidase-variable bacterial isolates. J Clin Microbiol 1982; 16:772–774 [View Article]
    [Google Scholar]
  5. Xu P, Li W-J, Tang S-K, Zhang Y-Q, Chen G-Z et al. Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family 'Oxalobacteraceae' isolated from China. Int J Syst Evol Microbiol 2005; 55:1149–1153 [View Article]
    [Google Scholar]
  6. Teather RM, Wood PJ. Use of Congo red–polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol 1982; 43:777–780 [View Article]
    [Google Scholar]
  7. Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994
    [Google Scholar]
  8. Lane DJ. 16S/23S rRNA Sequencing. In Stackebrandt E, Goodfellow M. (editors) Nucleic Acid Techniques in Bacterial Systematics New York: Wiley; 1991 pp 115–175
    [Google Scholar]
  9. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article]
    [Google Scholar]
  10. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22:4673–4680 [View Article]
    [Google Scholar]
  11. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article]
    [Google Scholar]
  12. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI inc; 1990
    [Google Scholar]
  13. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  14. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 1982; 5:2359–2367 [View Article]
    [Google Scholar]
  15. Schleifer KH, Kandler O. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 1972; 36:407–477 [View Article]
    [Google Scholar]
  16. Yoon JH, Kim H, Kim SB, Kim HJ, Kim WY et al. Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 1996; 46:502–505 [View Article]
    [Google Scholar]
  17. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984; 25:125–128 [View Article]
    [Google Scholar]
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