1887

Abstract

A bacterium that was Gram-staining-positive, facultatively anaerobic, non-motile, rod- or filamentous-shaped, designated as strain 2JSPR-7, was isolated from a gut of larvae of which were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea. 2JSPR-7 had the highest 16S rRNA gene sequence similarity to XIL08 (98.1 %), NBRC 107786 (97.8 %) and DSM 15894 (97.5 %). Optimum growth conditions were at 28–30 °C, pH 7–8 and 0 % salt concentration. The cellular fatty acids mainly consisted of anteiso-C, C and C. The polar lipids were diphosphatidylglycerol, four unidentified phospholipids and two unidentified glycophospholipids. The major menaquinones were MK-8(H) and MK-9(H). The peptidoglycan structure was suggested to be the type A3 (A11.14) -Lys-Ser with the presence of -Ala, -Ala, -Glu, -Ser and -Lys. Whole cell sugars were rhamnose, ribose and glucose. The DNA G+C content was 72.7 mol%. We encountered difficulty in selecting a suitable genus to accommodate strain 2JSPR-7 from any of the genera , and based on the polyphasic approach including phylogenetic and phenotypic characterization. Therefore, it is proposed to combine the genera and with the genus considering the priority of publication and to classify strain 2JSPR-7 in the genus as sp. nov. The type strain of the novel species is 2JSPR-7 (=KACC 19330=NBRC 113052). In addition, the description of the genus is emended, and and are reclassified as comb. nov. and comb. nov., respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004001
2020-01-24
2020-02-28
Loading full text...

Full text loading...

References

  1. Stackebrandt E, Rainey FA, Ward-Rainey NL. Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 1997;47:479–491 [CrossRef]
    [Google Scholar]
  2. Schumann P, Weiss N, Stackebrandt E. Reclassification of Cellulomonas cellulans (Stackebrandt and Keddie 1986) as Cellulosimicrobium cellulans gen. nov., comb. nov. Int J Syst Evol Microbiol 2001;51:1007–1010 [CrossRef]
    [Google Scholar]
  3. Stackebrandt E, Schumann P, Cui XL. Reclassification of Cellulosimicrobium variabile Bakalidou et al. 2002 as Isoptericola variabilis gen. nov., comb. nov. Int J Syst Evol Microbiol 2004;54:685–688 [CrossRef]
    [Google Scholar]
  4. Nishijima M, Tazato N, Handa Y, Umekawa N, Kigawa R et al. Krasilnikoviella muralis gen. nov., sp. nov., a member of the family Promicromonosporaceae, isolated from the Takamatsuzuka Tumulus stone chamber interior and reclassification of Promicromonospora flava as Krasilnikoviella flava comb. nov. Int J Syst Evol Microbiol 2017;67:294–300 [CrossRef]
    [Google Scholar]
  5. Cui X, Schumann P, Stackebrandt E, Kroppenstedt RM, Pukall R et al. Myceligenerans xiligouense gen. nov., sp. nov., a novel hyphae-forming member of the family Promicromonosporaceae. Int J Syst Evol Microbiol 2004;54:1287–1293 [CrossRef]
    [Google Scholar]
  6. Krasil'nikov NA, Kalakoutskii LV, Kirillova NF. A new genus of Actinomycetales, Promicromonospora, gen. nov. Bulletin of the Academy of Sciences USSR 1961;1:107–112
    [Google Scholar]
  7. Rivas R, Trujillo ME, Schumann P, Kroppenstedt RM, Sánchez M et al. Xylanibacterium ulmi gen. nov., sp. nov., a novel xylanolytic member of the family Promicromonosporaceae. Int J Syst Evol Microbiol 2004;54:557–561 [CrossRef]
    [Google Scholar]
  8. Stackebrandt E, Schumann P. Reclassification of Promicromonospora pachnodae Cazemier et al. 2004 as Xylanimicrobium pachnodae gen. nov., comb. nov. Int J Syst Evol Microbiol 2004;54:1383–1386 [CrossRef]
    [Google Scholar]
  9. Rivas R, Sánchez M, Trujillo ME, Zurdo-Piñeiro JL, Mateos PF et al. Xylanimonas cellulosilytica gen. nov., sp. nov., a xylanolytic bacterium isolated from a decayed tree (Ulmus nigra). Int J Syst Evol Microbiol 2003;53:99–103 [CrossRef]
    [Google Scholar]
  10. Felske A, Rheims H, Wolterink A, Stackebrandt E, Akkermans ADL. Ribosome analysis reveals prominent activity of an uncultured member of the class Actinobacteria in grassland soils. Microbiology 1997;143:2983–2989 [CrossRef]
    [Google Scholar]
  11. Lagesen K, Hallin P, Rødland EA, Stærfeldt H-H, Rognes T et al. RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res 2007;35:3100–3108 [CrossRef]
    [Google Scholar]
  12. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef]
    [Google Scholar]
  13. Pruesse E, Peplies J, Glöckner FO. Sina: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 2012;28:1823–1829 [CrossRef]
    [Google Scholar]
  14. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425
    [Google Scholar]
  15. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  16. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef]
    [Google Scholar]
  17. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870–1874 [CrossRef]
    [Google Scholar]
  18. Tatusova T, DiCuccio M, Badretdin A, Chetvernin V, Nawrocki EP et al. NCBI prokaryotic genome annotation pipeline. Nucleic Acids Res 2016;44:6614–6624 [CrossRef]
    [Google Scholar]
  19. Auch AF, von Jan M, Klenk H-P, Göker M. Digital DNA–DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2010;2:117–134 [CrossRef]
    [Google Scholar]
  20. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013;14:60 [CrossRef]
    [Google Scholar]
  21. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018;68:461–466 [CrossRef]
    [Google Scholar]
  22. Smibert R, Krieg NR.Phenotypic characterization In Gerhardt P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994; pp607–654
    [Google Scholar]
  23. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  24. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  25. Staneck JL, Roberts GD. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 1974;28:226–231 [CrossRef]
    [Google Scholar]
  26. Hamada M, Tamura T, Yamamura H, Suzuki K-ichiro, Hayakawa M et al. Lysinimicrobium mangrovi gen. nov., sp. nov., an actinobacterium isolated from the rhizosphere of a mangrove. Int J Syst Evol Microbiol 2012;62:1731–1735 [CrossRef]
    [Google Scholar]
  27. Kämpfer P, Glaeser SP, Kloepper JW, Hu C-H, McInroy JA et al. Isoptericola cucumis sp. nov., isolated from the root tissue of cucumber (Cucumis sativus). Int J Syst Evol Microbiol 2016;66:2784–2788 [CrossRef]
    [Google Scholar]
  28. Bakalidou A, Kämpfer P, Berchtold M, Kuhnigk T, Wenzel M et al. Cellulosimicrobium variabile sp. nov., a cellulolytic bacterium from the hindgut of the termite Mastotermes darwiniensis. Int J Syst Evol Microbiol 2002;52:1185–1192
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004001
Loading
/content/journal/ijsem/10.1099/ijsem.0.004001
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error