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Volume 70, Issue 2

sp. nov., a novel endophytic actinomycete isolated from the root of Free

Abstract

Strain CR1-09, an actinomycete isolated from the root of , was taxonomically studied based upon polyphasic approaches. The isolate formed a pair of ovular to circular, smooth-surfaced spores on short sporophores alternately branched from aerial mycelia. It contained -diaminopimelic acid in cell wall peptidoglycans. The major menaquinones were MK-9 (H) and MK-9 (H). The predominant cellular fatty acids were iso-C and C. The polar lipids profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxyl phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, and unidentified ninhydrin positive phosphoglycolipids. Strain CR1-09 showed the highest 16S rRNA gene sequence similarity with DSM 104650 (99.5 %). Based on the polyphasic approach, DNA–DNA relatedness and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus , for which the name is proposed. The type strain is strain CR1-09 (=JCM 30045=TISTR 2273).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): 16SrRNA gene , Catharanthus roseus , endophytic actinomycetes , genome , Microbispora and polyphasic taxonomy
Funding
This study was supported by the:
  • the Grant for International Research Integration, Research Pyramid, Ratchadaphiseksomphot Endowment Fund (Award GCURP_58_01_33_01)
    • Principle Award Recipient: Somboon Tanasupawat
  • Royal Golden Jubilee (RGJ) Ph.D. Programme (Award 5 . C . CU / 59 / I . 1)
    • Principle Award Recipient: Nattaporn Klykleung
© 2020 The Authors
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2019-11-15
2023-11-28
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Microbispora catharanthi sp. nov., a novel endophytic actinomycete isolated from the root of Catharanthus roseus
Int J Syst Evol Microbiol 70, 964 (2020); https://doi.org/10.1099/ijsem.0.003858
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