%0 Journal Article %A Klykleung, Nattaporn %A Yuki, Masahiro %A Kudo, Takuji %A Ohkuma, Moriya %A Phongsopitanun, Wongsakorn %A Pittayakhajonwut, Pattama %A Tanasupawat, Somboon %T Microbispora catharanthi sp. nov., a novel endophytic actinomycete isolated from the root of Catharanthus roseus %D 2020 %J International Journal of Systematic and Evolutionary Microbiology, %V 70 %N 2 %P 964-970 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.003858 %K endophytic actinomycetes %K genome %K Microbispora %K Catharanthus roseus %K polyphasic taxonomy %K 16SrRNA gene %I Microbiology Society, %X Strain CR1-09T, an actinomycete isolated from the root of Catharanthus roseus, was taxonomically studied based upon polyphasic approaches. The isolate formed a pair of ovular to circular, smooth-surfaced spores on short sporophores alternately branched from aerial mycelia. It contained meso-diaminopimelic acid in cell wall peptidoglycans. The major menaquinones were MK-9 (H4) and MK-9 (H2). The predominant cellular fatty acids were iso-C16 : 0 and C16 : 0. The polar lipids profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxyl phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, and unidentified ninhydrin positive phosphoglycolipids. Strain CR1-09T showed the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %). Based on the polyphasic approach, DNA–DNA relatedness and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Microbispora , for which the name Microbispora catharanthi is proposed. The type strain is strain CR1-09T (=JCM 30045T=TISTR 2273T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003858