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Volume 70, Issue 3

sp. nov., isolated from ginseng cultivation soil Free

Abstract

A Gram-stain-negative, strictly aerobic, motile, ivory-coloured and rod-shaped bacterium (designated Gsoil 520) isolated from ginseng cultivation soil was characterized by using a polyphasic approach to clarify its taxonomic position. Strain Gsoil 520 was observed to grow optimally at 30 °C and pH 7.0 on Reasoner's 2A agar medium. The results of phylogenetic analysis, based on 16S rRNA gene sequence similarities, indicated that Gsoil 520 belongs to the genus of the family and was most closely related to E84 (98.0 %), Yak96B (97.7 %), J1 (97.7 %) and IPL18 (96.8 %). The complete genome of strain Gsoil 520 is a presumptive circular chromosome of 4 480 314 base pairs having G+C content of 63.7 mol%. A total of 4 354 genes, 4 303 CDS and 43 rRNA genes were assigned a putative function. The major isoprenoid quinone was Q-10. The main polar lipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified aminolipids (AL1 and AL3). The predominant fatty acids of strain Gsoil 520 were Cω7 11-methyl, C and Cω7/Cω6 (summed feature 8) supporting the affiliation of strain Gsoil 520 to the genus . The low values of DNA–DNA hybridization distinguished strain Gsoil 520 from the recognized species of the genus . Thus, the novel isolate represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain Gsoil 520 (=KACC 19440=LMG 30329).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Devosia ginsengisoli , genome sequencing (complete and 16S rRNA) , Hyphomicrobiaceae and polyphasic taxomony
Funding
This study was supported by the:
  • This work was supported by the project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources (NIBR) under the Ministry of Environment and Rural Development Administration, Republic of Korea, and by a grant from the Korea Research Institute of Bioscience & Biotechnology (KRIBB) Research Initiative Program.
    • Principle Award Recipient: Wan-Taek Im
© 2020 The Authors
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2020-01-16
2024-05-14
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Devosia ginsengisoli sp. nov., isolated from ginseng cultivation soil
Int J Syst Evol Microbiol 70, 1489 (2020); https://doi.org/10.1099/ijsem.0.003843
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