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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 2

sp. nov., a new species isolated from faeces of a red fox in Spain Free

Abstract

A facultative anaerobic, chemoheterotrophic, endospore-forming, Gram-stain-positive rod, designated as strain Z8, was isolated from red fox () faeces sampled at Tablas de Daimiel National Park, Ciudad Real, Spain. Strain Z8 grew at 0–37 °C (optimum, 28 °C), in the presence of 0–5.5 % (w/v) NaCl (2.5 %, w/v) and at pH 6–10 (pH 7). The strain was motile and positive for catalase, oxidase, HS and siderophore production, acid and alkaline phosphatases, and -acetylglucosamine, adipic acid and malate assimilation. It hydrolysed starch, DNA, -tyrosine, Tween 20, Tween 80 and lecithovitellin. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain Z8 is a member of the genus , showing high sequence similarity to NEAU-3TGS17 (99.2 %) and NHI-2T (99.1 %), and around 98 % to other known species of the genus . Digital DNA–DNA hybridization and average nucleotide identity values were lower than 24 and 79 %, respectively, with the most related species. G+C content was 35.9 mol%. The major cellular fatty acids of strain Z8 were iso-C, iso-C and anteiso-C. The novel strain contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol as predominant polar lipids, and the main respiratory isoprenoid quinone was MK-8. Based on the 16S rRNA phylogenetic analysis, together with MLSA ( and ), phylogenomic, chemotaxonomic and phenotypic results, we demonstrate that strain Z8 represents a novel species of the genus for which the name sp. nov., is proposed. The type strain is Z8 (=CECT 9721=LMG 31001).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): chemotaxonomy , genome , phylogenetic , polyphasic taxonomy , Psychrobacillus vulpis sp. nov. and red fox
Funding
This study was supported by the:
  • Spanish Ministerio de Educación y Ciencia (Award AGL-2015-68806-R)
    • Principle Award Recipient: Inmaculada Llamas Company
© 2020 The Authors
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2019-12-13
2025-01-24
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Psychrobacillus vulpis sp. nov., a new species isolated from faeces of a red fox in Spain
Int J Syst Evol Microbiol 70, 882 (2020); https://doi.org/10.1099/ijsem.0.003840
/content/journal/ijsem/10.1099/ijsem.0.003840
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