@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.003840, author = "Rodríguez, Miguel and Reina, José Carlos and Béjar, Victoria and Llamas, Inmaculada", title = "Psychrobacillus vulpis sp. nov., a new species isolated from faeces of a red fox in Spain", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "2", pages = "882-888", doi = "https://doi.org/10.1099/ijsem.0.003840", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003840", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "chemotaxonomy", keywords = "Psychrobacillus vulpis sp. nov.", keywords = "polyphasic taxonomy", keywords = "phylogenetic", keywords = "genome", keywords = "red fox", abstract = "A facultative anaerobic, chemoheterotrophic, endospore-forming, Gram-stain-positive rod, designated as strain Z8T, was isolated from red fox (Vulpes vulpes) faeces sampled at Tablas de Daimiel National Park, Ciudad Real, Spain. Strain Z8T grew at 0–37 °C (optimum, 28 °C), in the presence of 0–5.5 % (w/v) NaCl (2.5 %, w/v) and at pH 6–10 (pH 7). The strain was motile and positive for catalase, oxidase, H2S and siderophore production, acid and alkaline phosphatases, and N-acetylglucosamine, adipic acid and malate assimilation. It hydrolysed starch, DNA, l-tyrosine, Tween 20, Tween 80 and lecithovitellin. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain Z8T is a member of the genus Psychrobacillus , showing high sequence similarity to Psychrobacillus lasiicapitis NEAU-3TGS17T (99.2 %) and Psychrobacillus soli NHI-2TT (99.1 %), and around 98 % to other known species of the genus Psychrobacillus . Digital DNA–DNA hybridization and average nucleotide identity values were lower than 24 and 79 %, respectively, with the most related species. In silico G+C content was 35.9 mol%. The major cellular fatty acids of strain Z8T were iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0. The novel strain contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol as predominant polar lipids, and the main respiratory isoprenoid quinone was MK-8. Based on the 16S rRNA phylogenetic analysis, together with MLSA (recA, rpoB and gyrB), phylogenomic, chemotaxonomic and phenotypic results, we demonstrate that strain Z8T represents a novel species of the genus Psychrobacillus, for which the name Psychrobacillus vulpis sp. nov., is proposed. The type strain is Z8T (=CECT 9721T=LMG 31001T).", }